Protein levels in mouse tissues or cells were determined by western blotting using the following antibodies: anti‐TPPP2 (Abcam, 121215; 1:1000), anti‐green fluorescent protein (GFP) (EnoGene, E12‐026‐4; 1:3000), anti‐β‐ACTIN (Merck Millipore, MAB1501; 1:7500), anti‐Caspase3 (Proteintech, 19677‐1‐AP; 1:500), anti‐Bax (Proteintech, 50599‐2‐Ig; 1:500), anti‐Bcl‐2 (Proteintech, 12789‐1‐AP; 1:500) and anti‐Eef1b (Proteintech, 10483‐1‐P; 1:1000).
Mab1501
Manufactured by Proteintech
Sourced in United Kingdom
MAB1501 is a mouse monoclonal antibody. It is designed for use in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti caspase 3, by Proteintech (1 mentions)
Anti bcl 2, by Proteintech (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti bax, by Proteintech (1 mentions)
Enhanced chemiluminescence detection kit, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Proteintech (1 mentions)
Mjf r20, by Abcam (1 mentions)
A0545, by Merck Group (1 mentions)
Gtx113340 10, by GeneTex (1 mentions)
Fusion fx, by Vilber (1 mentions)
Immobilon p pdvf membrane, by Merck Group (1 mentions)
Immobilon western hrp substrate, by Merck Group (1 mentions)
Radiance plus, by Azure Biosystems (1 mentions)
4 protocols using mab1501
1
Western Blotting of Protein Levels
2
Immunoblotting Protocol for Protein Detection
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Immunoblotting was performed as described by Chiang et al. (2014) using an enhanced chemiluminescence detection kit (Thermo Scientific).11 (link) Antibodies used were against the following proteins or epitopes: CstF64 (Abcam, ab72297), MRJ (Abnova, H00010049-A01), RSV F (Santa Cruz Biotechnology, sc-101362), RSV multiple proteins (Abcam, ab20745), HA (Covance, 16B12), actin (EMD Millipore, MAB1501), and GAPDH (Proteintech, 10494-1-AP). Horseradish peroxidase (HRP)-conjugated secondary antibodies included anti-mouse immunoglobulin G (IgG; SeraCare, 5210-0183) and anti-rabbit IgG (GeneTex, GTX213110-01)
3
Generation of Antibodies for Protein Detection
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Anti-Aux and anti-INPP5F polyclonal antibodies were raised against recombinant GST-tagged Aux (720–1165 aa) and GST-tagged INPP5F (744–1000 aa) produced in the Escherichia coli strain Rosetta 2 (Novagen, Merck, Darmstadt, Germany). Rabbit anti-RME-8 polyclonal antibody was raised against a mixture of synthetic peptides (C-ISTYNPDKLDLTNRWS-coNH2 and C-KDQRHDLFIADTTIRGY-coNH2) and purified through affinity chromatography (Japan Bio Services, Asaka, Japan). Rabbit anti-Chc polyclonal antibody was raised against a synthetic peptide (DDSTEHKNIIQMEPQLMC; Cosmo Bio, Tokyo, Japan).
The following primary antibodies were used for western blotting: anti-GFP (1:5000; 598, MBL, Tokyo, Japan), anti-pRab (1:1000; MJF-R20, Abcam, Cambridge, UK), anti-Lrrk (1:2000; in-house, 2136013), anti-Aux (1:1000; in-house, R1), anti-INPP5F (1:1000; in-house, GP-C2), anti-RME-8 (1:1000, in-house, 1738071), anti-Actin (1:10000; Millipore, MAB1501), anti-GAPDH (1:1000; Proteintech, 1E6D9), and anti-Tubulin (1:5000; Sigma-Aldrich, DM1A).
The following primary antibodies were used for western blotting: anti-GFP (1:5000; 598, MBL, Tokyo, Japan), anti-pRab (1:1000; MJF-R20, Abcam, Cambridge, UK), anti-Lrrk (1:2000; in-house, 2136013), anti-Aux (1:1000; in-house, R1), anti-INPP5F (1:1000; in-house, GP-C2), anti-RME-8 (1:1000, in-house, 1738071), anti-Actin (1:10000; Millipore, MAB1501), anti-GAPDH (1:1000; Proteintech, 1E6D9), and anti-Tubulin (1:5000; Sigma-Aldrich, DM1A).
4
Western Blot Analysis of Arf Proteins
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Cell lysates were separated by SDS-gel electrophoresis (15% polyacrylamide for Arfs) and transferred to Immobilon-P PDVF membranes (Millipore). Membranes were blocked with TBST (20 mM Tris, 150 mM NaCl, pH 7.6, 0.1% Tween20) with 3% non-fat dry milk for 1 h and incubated with primary antibody in TBST with 1% milk over night at 4°C: anti-Arf1 (1:2500, Abnova MAB10011), anti-Arf3 (1:1000, BD Bioscience 610784), anti-Arf4 (1:1000, Proteintech 11673-1-AP), anti-Arf5 (1:750, Abnova H00000381-M01), anti-actin (1:100000, Sigma-Aldrich MAB1501), anti-calreticulin (1:2500, Proteintech 27298-1-AP), anti-Grp78/Bip (1:10000, Genetex GTX113340-10). After washing, the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk. Chemiluminescence signals were detected using Immobilon Western HRP Substrate (Millipore) or Radiance Plus (Azure Biosystems) and imaged using a FusionFX (Vilber Lourmat).
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