Endothelial growth supplement

Manufactured by Merck Group

Sourced in United States

Endothelial growth supplement is a laboratory reagent designed to support the in vitro culture and growth of endothelial cells. It contains a blend of essential growth factors and supplements required for the optimal proliferation and maintenance of endothelial cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Heparin, by Merck Group (3 mentions) Murine recombinant interferon γ, by R&D Systems (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Euroclone (1 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) Alpha mem, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions) F 12k medium, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Medium 199, by Thermo Fisher Scientific (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Escherichia coli lipopolysaccharide lps, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

Close spelling variants of this product

Endothelial cell growth supplements Endothelial cell growth factor supplement Endothelial cell growth supplement from bovine pituitary Endothelial cell growth supplement from bovine neural tissue Endothelial cell growth supplement

6 protocols using endothelial growth supplement

Isolation and Characterization of Murine Vascular Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary SMCs were isolated from rat carotid arteries according to a method described previously ^{33 (link)} and maintained in DMEM supplemented with 10% FBS, and penicillin-streptomycin. Mouse aortic ECs were isolated from C57BL/6J immorto mice as described previously using anti-CD31 conjugated magnetic beads ^{34 (link)}. ECs were grown on gelatin-coated dishes in DMEM containing 10% FBS, 2 mM L-glutamine, 2 mM sodium pyruvate, 20 mM HEPES, 1% nonessential amino acids, 100 μg/ml streptomycin, 100 U/ml penicillin, freshly added heparin at 55 U/ml (Sigma, St. Louis, MO), endothelial growth supplement 100 μg/ml (Sigma), and the murine recombinant interferon-γ (R&D, Minneapolis, MN) at 44 U/ml. The endothelial identity was confirmed by FACS analysis for CD31, VE-cadherin, and B4-lectin.

Ren J., Zhou T., Pilli V.S., Phan N., Wang Q., Gupta K., Liu Z., Sheibani N, & Liu B. (2019). Novel Paracrine Functions of Smooth Muscle Cells in Supporting Endothelial Regeneration Following Arterial Injury. Circulation research, 124(8), 1253-1265.

+ Open protocol

+ Expand

Culturing Carcinoma and Glioblastoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEp-2 laryngeal carcinoma cells and U87MG human GBM cells were grown in Dulbecco’s Modified Eagle’s Medium (D-MEM, EuroClone S.p.A., Pero, Italy) enriched with 10% fetal bovine serum (FBS, EuroClone), 100 U/l mL of penicillin and 100 μg/mL of streptomycin. The cells were grown in a controlled atmosphere incubator (5% CO2, 90% humidity, temperature 37 °C). For the various steps, cells were detached with 10 mM EDTA and 0.25% trypsin in PBS, without calcium and magnesium, at pH 7.4. All treatments were performed in a microbiological safety cabinet.
For all experiments, the cells were seeded in Petri dishes or in 24-well plates, depending on the type of assay: (i) 24-well plates for all titration, inhibition, and competition experiments; (ii) 60 mm Petri dishes for western blot. Cells were seeded at a density of 2 × 10⁴ cm² cells. Twenty-four h after seeding, cells were treated with the An2-CNF1-H8, CNF1-H8 and wt CNF1 for different times depending on the experiment considered.
HBEC-5i cerebral microvascular endothelium cells (ATCC^® CRL3245™) were grown in 1% gelatin-coated culture flasks using DMEM:F12 with FBS 10% and 40 µg/mL endothelial growth supplement (Sigma–Aldrich, St. Louis, MO, USA).

Colarusso A., Maroccia Z., Parrilli E., Germinario E.A., Fortuna A., Loizzo S., Ricceri L., Tutino M.L., Fiorentini C, & Fabbri A. (2020). Cnf1 Variants Endowed with the Ability to Cross the Blood–Brain Barrier: A New Potential Therapeutic Strategy for Glioblastoma. Toxins, 12(5), 291.

+ Open protocol

+ Expand

Comparative Study of Dental and Bone Marrow MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two mesenchymal stem cell types were used in this study namely: Primary human dental pulp MSCs (DPSCs) and primary human bone marrow derived MSCs (HMSCs). DPSCs were a gift from Dr. Songtao Shi (University of Pennsylvania, School of Dental Medicine). HMSCs used in this study were purchased from ATCC. Both types of MSCs were cultured in MEM-alpha containing 20% fetal bovine serum (Gibco), 1% antibiotic-antimycotic solution (Gibco) and 1% L-Glutamine (Gibco). The cells were not passaged our used beyond passage 4. For experiments that required odontogenic differentiation of the cells, growth media was supplemented with 100 μg/ml ascorbic acid, 10 mM β-glycerophosphate and 10 mM dexamethasone.
Human umbilical vein endothelial cells were used to generate a pro-vascular ECM. These cells were cultured in F12K medium (Gibco) supplemented with 10% FBS (Gibco), heparin (Sigma) and endothelial growth supplement (Sigma) as per previously published protocols (Ravindran et al., 2004 (link)).

Huang C.C., Narayanan R., Warshawsky N, & Ravindran S. (2018). Dual ECM Biomimetic Scaffolds for Dental Pulp Regenerative Applications. Frontiers in Physiology, 9, 495.

+ Open protocol

+ Expand

Isolation and Characterization of Primary Trabecular Meshwork Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, the TM was dissected and treated with collagenase A (4 mg/ml) in DPBS for 2 hours. The tissue was then rinsed, centrifuged at 1,500× g and the cells contained in the pellet were maintained in Medium 199 (Gibco, MD, USA), containing 20% fetal bovine serum, heparin (90 ug/ml), endothelial growth supplement (Sigma, MO, USA), and 2 mM L-glutamine. Cells were maintained at 5% CO₂ and 37 °C. Before experimentation pTM were validated following published recommendations^{15 (link)}, including immunoreactivity to vimentin and absence of desmin expression and increased synthesis of myocilin upon dexamethasone treatment (see Supplemental Data).

Zhu W., Godwin C.R., Cheng L., Scheetz T.E, & Kuehn M.H. (2020). Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes. Scientific Reports, 10, 2905.

+ Open protocol

+ Expand

Choroid-RPE Explant Angiogenesis Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Choroid-RPE was dissected from C57BL/6j male and female 3-week-old mice and sectioned into 0.5- to 1-mm pieces as we previously described (Farnoodian et al., 2015 (link)). The 10 pieces per eye were placed into 35−mm culture dish coated with 0.5 ml of Matrigel (10 mg/ml; BD Biosciences) and allowed to harden (30 min at 37^oC). Endothelial cell growth medium (DMEM containing 10% FBS, 2 mmol/L L−glutamine, 2 mmol/L sodium pyruvate, 20 mmol/L HEPES, 1% non-essential amino acids, 100 μg/ml streptomycin, 100 U/ml penicillin, 55 U/ml heparin, and endothelial growth supplement 100 μg/ml (Sigma, St. Louis, MO, United States; E2759) was then added. After 48 h, the explants were fed every other day with the appropriate vehicle, 400 μM caffeine or 10 μM istradefylline. At 8 days, the explants were fixed with 4% paraformaldehyde and photographed (PFA; Electron Microscopy Sciences, Hatfield, PA, United States; 15710). The area of sprouting was quantified as previously described (Farnoodian et al., 2015 (link)).

Sorenson C.M., Song Y.S., Zaitoun I.S., Wang S., Hanna B.A., Darjatmoko S.R., Gurel Z., Fisk D.L., McDowell C.M., McAdams R.M, & Sheibani N. (2021). Caffeine Inhibits Choroidal Neovascularization Through Mitigation of Inflammatory and Angiogenesis Activities. Frontiers in Cell and Developmental Biology, 9, 737426.

+ Open protocol

+ Expand

Leukocyte Adhesion Assay in Immortal Murine Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mRECs, generated from immorto-mice as previously described [32 (link)], were grown in 24 well gelatin coated plates in DMEM containing 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA), 2 mM L-glutamine, 20 mM HEPES, 100 μg/ml streptomycin, 1% non-essential amino acids, 2mM sodium pyruvate, 100 U/ml penicillin, 55 U/ml of freshly added heparin at (Sigma), 100 μg/ml of endothelial growth supplement (Sigma), and 44 units/ml of murine recombinant interferon-γ (R & D, Minneapolis, MN) at 33°C with 5% CO₂. When mRECs were 80% confluent, they were treated with high glucose (HG; D-Glucose, 30 mM) or normo-osmotic control (LG, 5mM D-glucose + 25 mM L-glucose) for 1–3 days. As a positive control, mRECs were exposed to 1 μg/ml of the endotoxin Escherichia coli lipopolysaccharide (LPS; Sigma) for 24 hours. Thereafter, leukocytes (purified from the blood as described above) from WT or 12/15-LO^−/− mice were added to the mREC confluent monolayer and incubated for 90 minutes. After incubation, non-adherent leukocytes were carefully removed by gentle washing before measuring the activity of Myeloperoxidase (MPO), a specific marker for leukocytes, using Fluro MPO Myeloperoxidase Detection Kit (Catalog# MPO100-3, Cell Technology, Mountain View, CA,) according to the manufacturer’s instructions.

Ibrahim A.S., Saleh H., El-Shafey M., Hussein K.A., El-Masry K., Baban B., Sheibani N., Wang M.H., Tawfik A, & Al-Shabrawey M. (2017). Targeting of 12/15-Lipoxygenase in retinal endothelial cells, but not in monocytes/macrophages, attenuates high glucose-induced retinal leukostasis. Biochimica et biophysica acta, 1862(6), 636-645.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!