Percp conjugated anti mouse cd40

The following primary antibodies and the corresponding isotype controls were purchased from BD Pharmingen, Inc. (San Diego, CA, USA): PE‐conjugated anti‐mouse antibodies against CD3, CD11b, CD80, CD206 (clone M1), major histocompatibility complex class II (MHC II), NK1.1, programmed death‐ligand 1 (PD‐L1); FITC‐conjugated anti‐mouse antibodies against CD4, CD45; APC‐conjugated anti‐mouse antibodies against CD8, F4/80; APC‐Cy7‐conjugated anti‐mouse CD11c; PE‐Cy7‐conjugated anti‐mouse CD86; and PerCP‐conjugated anti‐mouse CD40. Anti‐ programmed cell death protein 1 (PD‐1) antibody (clone RPM1‐14) was purchased from Bio X Cell (Lebanon, NH, USA). Functional anti‐CD3 and anti‐CD28 monoclonal antibodies were obtained from Invitrogen (Carlsbad, CA, USA). Penicillin and streptomycin, the DCFH‐DA probe, Alexa Fluor® 488‐labeled goat anti‐rabbit IgG (H+L), Alexa Fluor® 555‐labeled donkey anti‐mouse IgG (H+L), and HRP‐conjugated goat anti‐mouse IgG (H+L) were obtained from Beyotime (Haimen, Jiangsu, China). PX‐478 and GM6001 were obtained from ApexBio (Houston, TX, USA). Matrigel and macrophage colony‐stimulating factor (MCSF) were purchased from Sigma (St. Louis, MO, USA) and GenScript (Nanjing, Jiangsu, China), respectively.