Lmplfln

Manufactured by Olympus

Sourced in United States, Japan

The LMPLFLN is a laboratory microscope objective lens from Olympus. It is designed for low magnification applications. The objective lens provides high numerical aperture and long working distance to facilitate wide-field observation of specimens.

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Lab products found in correlation

Infinity 2, by OceanOptics (3 mentions) Qe65000, by Olympus (3 mentions) Ledmt1e, by Thorlabs (2 mentions) Dcc1545m, by Thorlabs (2 mentions) Emccd camera, by Horiba (2 mentions) Triax 320 spectrometer, by Horiba (2 mentions) Ace 1, by Schott (1 mentions) Senterra 2, by Bruker (1 mentions) Afc3011c, by Rigol (1 mentions) Dg4202, by Rigol (1 mentions) Kinesis, by Thorlabs (1 mentions) Orca er c4742 80, by Hamamatsu Photonics (1 mentions)

11 protocols using lmplfln

Plasmonic Microbubble Imaging Setup

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Figure 1 shows the
experimental setup for the plasmonic microbubble imaging. The gold
nanoparticle decorated sample is placed in a glass cuvette, submerged
in a liquid and irradiated by a 300 mW, 532 nm continuous wave laser
(Cobolt Samba). The light intensity at the substrate is controlled
via a halfwave plate and a polarizer and measured by a photodiode
power sensor (S130C, ThorLabs). An acoustic-optic modulator acts as
a triggerable shutter. The laser spot diameter is 10 μm, and
the intensity can be varied between 0 and 200 mW. Laser pulses of
400 μs were generated and controlled by a pulse/delay generator
(BNC model 565) in order to study the short-term dynamics of the microbubbles.
Two high-speed cameras were installed in the setup, one for the top
view and another for the side view. The top view camera (SA7) is equipped
with a 5× long working distance objective (LMPLFLN, Olympus).
The side view camera (Photron SAZ) can be equipped with two long working
distance objectives, 10× (LMPLFLN, Olympus) or 20× (SLMPLN,
Olympus), and can be operated at frame rates up to 1200 kfps. Two
light sources, a Sumita LS-M350 and a Schott ACE I, provided back
light illumination for the high-speed cameras.

Detert M., Zeng B., Wang Y., Le The H., Zandvliet H.J, & Lohse D. (2019). Plasmonic Bubble Nucleation in Binary Liquids. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 124(4), 2591-2597.

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OpenSPIM Imaging of Cleared Samples

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Observation was performed with a home-built OpenSPIM system equipped with a 10 × objective lens (Olympus LMPLFLN, NA = 0.25, WD = 21 mm). Cleared agarose-embedded samples were positioned under a 4D stage in front of the objective in the mounting medium (Ub-2). The z step size was set 10 μm for samples (Fig. 4).

Chen L., Li G., Li Y., Li Y., Zhu H., Tang L., French P., McGinty J, & Ruan S. (2017). UbasM: An effective balanced optical clearing method for intact biomedical imaging. Scientific Reports, 7, 12218.

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Printing Setup for Controlled-Atmosphere Deposition

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The main components of the printing setup were (Supplementary Fig. 16): a power source (B2962A, Keysight) for biasing the electrodes; mechanical relays (HE751, Littlefuse) for switching the high voltage between different electrodes; a printing nozzle; an electrically grounded substrate mounted on a three-axis piezo translation stage (QNP60XY-500-C-MP-TAS, QNP60Z-500-C-TAS, Aerotech); an optical microscope to observe the printing process. The piezo axes were mounted on an additional long-range axis (M112-1VG, PI) to enable stage translations larger than 500 μm. The nozzle was fixed to a manual three-axis micromanipulator-stage (HS 6, Märzhäuser Wetzlar GmbH) for coarse positioning. The mechanical relays were computer-controlled and interfaced with an Arduino UNO microcontroller board. The microscope was built from a ×50 objective-lens (LMPLFLN, Olympus), a CMOS camera (DCC1545M, Thorlabs), and a blue LED light source (LEDMT1E, Thorlabs). The optical axis of the lens was inclined 60° to the substrate normal. To print at low oxygen levels (200–1000 ppm), an acrylic chamber enclosing the printer was flushed with argon gas (4.8, PanGas). Oxygen- and humidity-levels were monitored with a gas sensor (Module ISM-3, PBI Dansensor) and a humidity sensor (SHT31, Sensirion). Usual relative humidity and temperature during printing were 30–50% and 20–25 °C, respectively.

Reiser A., Lindén M., Rohner P., Marchand A., Galinski H., Sologubenko A.S., Wheeler J.M., Zenobi R., Poulikakos D, & Spolenak R. (2019). Multi-metal electrohydrodynamic redox 3D printing at the submicron scale. Nature Communications, 10, 1853.

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Raman Characterization of 2D Materials

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Raman characterization was done
using a confocal Raman microscope (Senterra II, Bruker). Flakes were
optically identified and Raman spectra at several locations within
the flake measured (for each flake we acquired between 6 and 23 individual
spectra, depending on the lateral size of the flake) using a long-working-distance
100× objective (Olympus, LMPLFLN). Each individual spectrum was
taken using 633 nm wavelength, power of 2 mW, 10 s acquisition time,
and 10 coacquisitions. The same conditions were used for the bulk
characterization. Spectra were individually analyzed using IgorPRO,
fitting Gaussian peaks to the Raman modes in the spectral range of
interest.

Quirós-Ovies R., Laborda M., Sabanés N.M., Martín-Pérez L., Silva S.M., Burzurí E., Sebastian V., Pérez E.M, & Santamaría J. (2023). Microwave-Driven Exfoliation of Bulk 2H-MoS2 after Acetonitrile Prewetting Produces Large-Area Ultrathin Flakes with Exceptionally High Yield. ACS Nano, 17(6), 5984-5993.

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Optical Characterization of Nanoparticle Plasmonic Cavities

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A customized microscope (Olympus BX51) with a halogen white light lamp, a charge-coupled device camera (Infinity 2), spectrometer (Ocean Optics QE65000), and a 100

\times

objective (NA 0.8 Olympus LMPLFLN) was used to capture optical DF images and spectra. All the DF spectra were integrated for 1 s and normalized using white light scattering of a standard diffuser. SERS spectra were recorded on the same microscope with a 633 nm laser for excitation, a 100

\times

objective (NA 0.9 Olympus LWD) and an Andor Newton EMCCD camera coupled to a Horiba Triax 320 spectrometer. For non-electrochemical SERS measurements on as-synthesized eNPoMs (in air), the laser power was set at 6

μ W

on 2 nm shell samples, and 3

μ W

on all other shell thickness NPoMs, for optimal signal-to-noise ratios and minimization of picocavity events. For electrochemical SERS measurements, the laser power was set to 10

μ W

for all samples. The integration time of all SERS measurements was 1 s if not otherwise specified. For non-electrochemical DF and SERS measurements, automated scans were performed using customized particle-tracking Python code.

Xiong Y., Chikkaraddy R., Readman C., Hu S., Xiong K., Peng J., Lin Q, & Baumberg J.J. (2024). Metal to insulator transition for conducting polymers in plasmonic nanogaps. Light, Science & Applications, 13, 3.

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Optical Dark-Field Imaging and SERS Analysis

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Optical dark-field
(DF) images and spectra of samples are acquired using a charge-coupled-device
camera (Infinity 2) and spectrometer (Ocean Optics QE65000) with numerical
aperture (NA) 0.8 100× objectives (Olympus LMPLFLN) in a customized
microscope (Olympus BX51). A halogen lamp is used as the white light
source. SERS measurements are recorded using the same microscope coupled
to a 633 nm laser at 100 μW with 1s integration time, and a
0.9 NA Olympus LWD objective for both excitation and collection. Spectra
are recorded by an Andor Newton EMCCD camera coupled to a Horiba Triax
320 spectrometer.

Peng J., Lin Q., Földes T., Jeong H.H., Xiong Y., Pitsalidis C., Malliaras G.G., Rosta E, & Baumberg J.J. (2022). In-Situ Spectro-Electrochemistry of Conductive Polymers Using Plasmonics to Reveal Doping Mechanisms. ACS Nano, 16(12), 21120-21128.

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Intravital Microscopy for Arteriolar Imaging

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Intravital imaging was performed on an optical microscope (OLYMPUS BX51), equipped with a 100 W halogen lamp, 1.1 N.A. Abbe condenser, and a long working distance ×50 objective (LMPLFLN, infinity-corrected optical system, working distance = 10.6 mm, NA = 0.5, Olympus, Center Valley, PA, USA). With this setup, the resolving power and depth of focus are 0.67 μm and 2.5 μm, respectively. A 335–610 nm bandpass filter (FGB37M, Thorlabs, Newton, NJ, USA) was used to enhance image contrast. After the muscle was exposed, stabilization of 20–30 min was allowed before measurements.
Video frames were recorded with a high-speed video camera (640 × 640 pixels, 2000 frames/s, shutter speed = 1/2000 s, Fastcam 60K-M1, Photron, San Diego, CA, USA). The spatial resolution of the images was 0.34 μm/pixel. The same arterioles chosen at baseline were followed throughout the experiment. Only arterioles in the range of 20 to 30 μm in diameter were used.

Corro-Hernández R., Aguila-Torres O., Rios A., Escalante B, & Santana-Solano J. (2022). Computer-assisted image analysis of agonist-mediated microvascular constriction response in mouse cremaster muscle. PLOS ONE, 17(11), e0277851.

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High-Speed Modulation of Monolayer WS2

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The high-speed modulation experiments (Fig. 5) were conducted on a home-built PL microscope system based on the Olympus microscope (BX43). During measurements, the constant 532 nm CW laser (Changchun Laser MW-ZGL-532) was focused on the WS₂ monolayer with the objective (Olympus LMPLFLN, 50×, NA = 0.5), and the time-resolved excitonic emission under the AC bias was measured via a TCSPC module (Swabian Instruments Time Tagger Ultra) which was synchronized with the function generator (Tektronix AFC3011C and Rigol DG4202) and the single photon avalanche diode (SPAD, MPD PD-100-CTE). All experiments were conducted in ambient environment at room temperature.

Zhu G., Zhang L., Li W., Shi X., Zou Z., Guo Q., Li X., Xu W., Jie J., Wang T., Du W, & Xiong Q. (2023). Room-temperature high-speed electrical modulation of excitonic distribution in a monolayer semiconductor. Nature Communications, 14, 6701.

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Optical Characterization of Single e-NPoMs

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Optical DF images and spectra of single eNPoMs were obtained using a charge-coupled device camera (Infinity 2) and spectrometer (Ocean Optics QE65000) with 100× objectives [Olympus LMPLFLN; numerical aperture (NA), 0.8] in a customized microscope (Olympus BX51). The white light source is a halogen lamp.

Peng J., Jeong H.H., Lin Q., Cormier S., Liang H.L., De Volder M.F., Vignolini S, & Baumberg J.J. (2019). Scalable electrochromic nanopixels using plasmonics. Science Advances, 5(5), eaaw2205.

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Electrohydrodynamic Printing Setup Characterization

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During deposition, the nozzle is kept stationary above the substrate and brought into focal plane of the optical system with a mechanical uniaxial stage (Z825B, Thorlabs), controlled with a motor controller (Kinesis, Thorlabs), in Y direction and two manual micrometre screws (Mitutoyo) in X and Z direction. The nozzle tip is observed with an optical microscope composed of a ×50 objective lens (LMPLFLN, Olympus) and a CMOS camera (DCC1545M, Thorlabs), illuminated from the opposite side using a green light source (LEDMT1E, Thorlabs). The lens is mounted at an inclination of 60° to the substrate normal. For printing, the substrate is moved by piezo stages in X, Y, and Z direction (QNPXY-500, QNP50Z-250, Ensemble QL controller, Aerotech). Stage translations in X and Y direction larger than 500 μm were enabled by additional long-range stages (M112-1VG, PI for Y direction, manual micrometre screw, Mitutoyo for X direction). A power source (B2962, Keysight) with triaxial cable connectors was used for polarizing the anodes. The metal wire, used as the sacrificial anode in the setup, was connected using a mechanical clamp. Piezo stages and voltage source were controlled through a custom Matlab script. The complete printing setup is mounted on a damped SmartTable (Newport) to provide a vibration-free environment.

Nydegger M., Pruška A., Galinski H., Zenobi R., Reiser A, & Spolenak R. (2022). Additive manufacturing of Zn with submicron resolution and its conversion into Zn/ZnO core–shell structures. Nanoscale, 14(46), 17418-17427.

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