Flow cytometry analysis was performed according to the manufacturer’s protocol. Cells were seeded in 6-well plates for 24 h, harvested and washed twice with cold PBS. Then, the cells were stained with PE-conjugated Annexin V and 7-AAD (BD Biosciences, San Jose, CA, USA) for 15 min at room temperature in the dark. Finally, the cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).
Pe conjugated annexin 5 and 7 aad
Manufactured by BD
Sourced in United States
PE-conjugated Annexin V and 7-AAD are fluorescently labeled reagents used in flow cytometry applications. Annexin V binds to phosphatidylserine, which is exposed on the surface of cells undergoing apoptosis. 7-AAD is a DNA-binding dye that can be used to assess cell viability. These reagents are commonly used together to identify and differentiate between viable, apoptotic, and necrotic cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (1 mentions)
Aria 2, by BD (1 mentions)
Aria 2 flow cytometer, by BD (1 mentions)
Pe cy7 conjugated sca1, by BD (1 mentions)
Apc conjugated c kit, by BD (1 mentions)
Pe annexin 5 apoptosis detection kit 1, by BD (1 mentions)
Kaluza 2, by Beckman Coulter (1 mentions)
Lsrfortessa x 20, by BD (1 mentions)
6 protocols using pe conjugated annexin 5 and 7 aad
1
Flow Cytometry Analysis of Apoptosis
2
Annexin V Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was assessed using flow cytometry. The harvested cells were washed twice with cold PBS and once with a binding buffer and incubated with PE-conjugated Annexin V and 7-AAD (BD Biosciences, Pharmingen, San Diego, CA, USA) for 15 min in the dark at 25°C. The cells were resuspended in a binding buffer and analyzed using a fluorescence-activated cell sorting Aria II flow cytometer (BD Biosciences). Each experiment was repeated thrice.
3
CAR19 and WT-iNK Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Target tumor cells were labeled with PKH67 lipophilic dye for 5 min at 37 °C and were subsequently incubated with the corresponding ratio of effector CAR19- and WT-iNK-like cells in a total concentration of 20,000 cells per well for 4 h at 37 °C in a round-bottom 96-well plate. After that, the cell mixture was harvested and stained with PE-conjugated annexin-V and 7-AAD (BD Biosciences, Franklin Lakes, NJ, USA) in Ca2+-rich buffer for 15 min at room temperature. Samples were analyzed immediately via FACS analysis. Total cell death of target tumor cells was defined as annexin-V and/or 7-AAD-positive cells in the PKH67-positive cell population.
4
Apoptosis Evaluation in SMMC-7721 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was assessed by flow cytometry analysis. SMMC-7721 cells were harvested and washed twice with cold PBS and once with binding buffer, and then incubated with PE-conjugated Annexin V and 7-AAD (BD Biosciences, Pharmingen, San Diego, CA) for 15 minutes in the dark at 25 °C. Cells were then resuspended in binding buffer and analyzed using a fluorescence-activated cell sorting (FACS) Aria II flow cytometer (BD Biosciences). Each experiment was repeated three times.
5
Multiparameter Flow Cytometry of Cell Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To monitor the expression of cell surface markers c-Kit, Sca-1, Mac-1, and Gr-1, cells were stained by APC-conjugated c-Kit, PE-cy7-conjugated Sca1, PE-conjugated Mac-1, and percp-cy5.5-conjugated Gr-1 antibodies purchased from BD Biosciences. To monitor apoptosis, cells were stained with PE-conjugated annexin V and 7-AAD using PE Annexin V Apoptosis Detection Kit I from BD Biosciences. Stained cells were evaluated using FACS Canto-II and data were analyzed by FlowJo_V10 software.
6
Apoptosis Assessment in H9C2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was assessed via an Annexin V-phycoerythrin (PE)/7-aminoactinomycin (AAD) assay. Briefly, after the indicated treatments, H9C2 cells were digested with trypsin, washed twice with cold PBS, collected, and suspended in Annexin V binding buffer. PE-conjugated Annexin V and 7-AAD (BD Biosciences, San Jose, CA, USA) were added to the cells. Following incubation, Annexin V binding buffer was added, and the cell suspensions were analysed by flow cytometry (BD LSRFortessa X-20; BD Biosciences) and data were analysed by Kaluza 2.0 (Beckman Coulter, Inc., Brea, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!