The antibodies against VEGFR3 (goat anti-mouse, R&D Systems (Minneapolis, MN) #AF743) and VEGFR2 (goat anti-mouse, R&D Systems #AF644) were used at a 1:50 dilution; the anti-LYVE (rabbit anti-mouse Abcam,Cambridge, MA) #ab14917) was used at 1:250; the anti-αSMA (mouse anti-mouse Dako,Santa Clara, CA) #M0851) was used at 1:100, and anti-PECAM/CD31 antibody (rat monoclonal, catalog #DIA-310; Dianova, Hamburg, Germany) was used at 1:250. Secondary antibodies were Donkey anti-rabbit 594 (#21207 ThermoFisher, Grand Island, NY); Donkey anti-goat 488 (Abcam #ab150129); Donkey anti-goat 546 (ThermoFisher #A11056); and Donkey anti-mouse 488 (Abcam #ab150105) were all used at a dilution of 1:250. Sections from a total of 3 pairs of A10ΔEC mice and littermate controls were analyzed per antibody, with at least one pair of littermates of each gender.
Donkey anti goat 488
Manufactured by Abcam
Sourced in United Kingdom
Donkey anti-goat 488 is a secondary antibody that binds to goat primary antibodies. It is conjugated with the fluorescent dye Alexa Fluor 488, which emits green fluorescence when excited.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Ab150105, by Abcam (1 mentions)
Af743, by R&D Systems (1 mentions)
Af644, by R&D Systems (1 mentions)
Anti α sma, by Agilent Technologies (1 mentions)
A11056, by Abcam (1 mentions)
Goat serum, by Thermo Fisher Scientific (1 mentions)
Donkey serum, by Thermo Fisher Scientific (1 mentions)
Donkey serum, by Merck Group (1 mentions)
Goat anti mouse alexa fluor 546, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade mounting media, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
2 protocols using donkey anti goat 488
1
Immunofluorescence Staining of Vascular Markers
2
Immunofluorescence Staining of Cells in PDMS Chambers
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Cells were fixed in the PDMS chambers for 20 min with 4% paraformaldehyde, blocked with 10% donkey serum (Merck Millipore, Germany) or 10% goat serum (Life Technologies), and permeabilized with 0.1% Triton-X. The PDMS substrate was cut into smaller pieces to be incubated overnight at 4 °C with primary antibodies diluted in 1% donkey serum or 1% goat serum (Supplementary Table 1 ). This was followed by incubation with appropriate secondary antibodies for 1 hr. The secondary antibodies that were used included goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 546 (Life Technologies), or donkey anti-goat 488 (Abcam, England, UK) at the concentration of 1:500. Cells were counterstained with DAPI (Sigma-Aldrich) for 20 min and mounted with Prolong Gold antifade mounting media (Life Technologies). Cell images were taken with Leica DM IRM inverted microscope (Leica, Germany) or confocal microscope (Zeiss LSM710, Germany).
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