Ab238941

Manufactured by Abcam

Ab238941 is a laboratory equipment product. It has a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab180191, by Abcam (2 mentions) Ab15580, by Abcam (2 mentions) Envision detection systems peroxidase dab, by Agilent Technologies (2 mentions)

2 protocols using ab238941

Immunohistochemical Analysis of Protein Expression

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Immunohistochemistry (IHC) staining was carried out using EnVision Detection Systems Peroxidase/DAB (DAKO, Shanghai, China) following the manufacturer’s recommendations. Slides containing the sections were stained with commercially available anti-USP9X (ab180191, 1:500, Abcam), anti-EGLN3 (ab238941, 1:500, Abcam) and anti-Ki67 (ab15580, 1:500, Abcam). Two experienced pathologists scored the stained tissues independently. By recording the percentage of positive staining (0 = negative, 1 ≤ 10%, 2 = 10–50%, 3 ≥ 50%) and staining intensity (0 = no, 1 = weak, 2 = moderate, 3 = strong) for each sample, immunoreactivity score (IRS) (0–9) was calculated by multiplying positive staining percentage with staining intensity. Low and high expression were defined according to the median IRS.

Chen W., Song J., Liu S., Tang B., Shen L., Zhu J., Fang S., Wu F., Zheng L., Qiu R., Chen C., Gao Y., Tu J., Zhao Z, & Ji J. (2021). USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1Bβ via deubiquitinating EGLN3. Journal of Biomedical Science, 28, 44.

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Immunohistochemical Analysis of Protein Targets

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Immunohistochemistry (IHC) staining was carried out using EnVision Detection Systems Peroxidase/DAB (DAKO, Shanghai, China) following the manufacturer's recommendations. Slides containing the sections were stained with commercially available anti-USP9X (ab180191, 1:500, Abcam), anti-EGLN3 (ab238941, 1:500, Abcam) and anti-Ki67 (ab15580, 1:500, Abcam). Two experienced pathologists scored the stained tissues independently. By recording the percentage of positive staining (0 = negative, 1 ≤ 10%, 2 = 10-50%, 3 ≥ 50%) and staining intensity (0 = no, 1 = weak, 2 = moderate, 3 = strong) for each sample, immunoreactivity score (IRS) (0-9) was calculated by multiplying positive staining percentage with staining intensity. Low and high expression were de ned according to the median IRS.

Chen W., Song J., Liu S., Tang B., Shen L., Zhu J., Fang S., Wu F., Zheng L., Qiu R., Chen C., Gao Y., Tu J., Zhi Z., & Ji J. (2020). Stabilization of EGLN3 by USP9X Contributes to Antineoplastic Function in Cholangiocarcinoma.

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