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Human cxcl13 quantikine elisa kit

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The Human CXCL13 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human CXCL13 levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Dynabeads cd8, by STEMCELL (2 mentions) Human high sensitivity cytokine premixed kit b, by R&D Systems (2 mentions) Bio plex 200 array system, by Bio-Rad (2 mentions) Anti ttf1, by Abcam (1 mentions) Emt antibody sampler kit, by Cell Signaling Technology (1 mentions) Mouse anti human β actin antibody, by Merck Group (1 mentions) Mouse cxcl12 quantikine elisa kit, by R&D Systems (1 mentions) Alexa fluor 488 donkey anti goat igg h l, by Thermo Fisher Scientific (1 mentions) Mouse cxcl13 quantikine elisa kit, by R&D Systems (1 mentions) Alexa fluor 647 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

4 protocols using human cxcl13 quantikine elisa kit

1

Cytokine Profiling of HIV-specific CD8+ T cells

Cited 1 time
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One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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2

CXCL13 Quantitation in Plasma

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CXCL13 was detected in plasma using the Human CXCL13 Quantikine ELISA Kit (R&D Systems) as described by the manufacturer.
Richardson S.I., Chung A.W., Natarajan H., Mabvakure B., Mkhize N.N., Garrett N., Abdool Karim S., Moore P.L., Ackerman M.E., Alter G, & Morris L. (2018). HIV-specific Fc effector function early in infection predicts the development of broadly neutralizing antibodies. PLoS Pathogens, 14(4), e1006987.
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3

Cytokine Profiling of HIV-specific CD8+ T cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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4

Investigating CXCL13 and CXCR5 in Lung Disease

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The antibodies used in this work were: recombinant human CXCL13, human CXCL13 Quantikine ELISA Kit, mouse CXCL13 Quantikine ELISA Kit, mouse CXCL12 Quantikine ELISA Kit, mouse anti-human CXCR5-PE mAb, goat anti-human CXCL13 polyclonal Ab, goat anti-mouse CXCL13 polyclonal Ab (R&D, Minneapolis, MN), rat anti-mouse CD68-PE, rat anti-mouse CD45-APC, rat anti-mouse CD4-PerCP-CY5.5, rat anti-mouse CD19-FITC, rat anti-mouse CXCR5-PE/CY7 mAb (Biolegend, San Diego, CA), mouse anti-human CD68 mAb (Dako, Glostrup, Denmark), rabbit anti-mouse SPP1 polyclonal Ab (Proteintech, Chicago, IL), anti-TTF1 (Abcam, Cambridge, UK), rabbit anti-mouse E-Cadherin mAb, EMT Antibody Sampler Kit (Cell Signaling Technology, Beverly, MA), rabbit anti-human AhR polyclonal Ab, goat anti-human Lamin B polyclonal Ab, mouse anti-human ɑ-tubulin mAb (Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-human β-actin antibody (Sigma, St. Louis, MO), Alexa Fluor 488 Donkey anti-Goat IgG (H+L), Alexa Fluor 555 Donkey Anti-Mouse IgG (H+L), Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L) (Life Technology, Thermo Fisher Scientific, Basingstoke, UK), and mouse SPP1 ELISA Kit (Shanghai GenePharma, Shanghai, China). BaP and DEX were purchased from Sigma.
Wang G.Z., Cheng X., Zhou B., Wen Z.S., Huang Y.C., Chen H.B., Li G.F., Huang Z.L., Zhou Y.C., Feng L., Wei M.M., Qu L.W., Cao Y, & Zhou G.B. (2015). The chemokine CXCL13 in lung cancers associated with environmental polycyclic aromatic hydrocarbons pollution. eLife, 4, e09419.
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