Mouse joint and knee tissues were sectioned into 5 μm thick samples. Antigen retrieval was performed by incubating the section with 0.1% trypsin for 40 min at 37°C or with citrate buffer (pH 6.0) for 20 min at 95°C. Anti‐CD90.2 (140 301; BioLegend), anti‐CCL2 (ab25124, Abcam, Cambridge, UK), anti‐ CXCL1 (LS‐B13384; LifeSpan BioSciences, Inc. Seattle, WA, USA), anti‐IL‐6 (ab9324, Abcam), anti‐COX2 (SC‐1745, Santa Cruz Biotechnology, Dallas, TX, USA), anti‐MMP3 (ab52915, Abcam), anti‐MMP13 (ab51072, Abcam), anti‐elastase (Ab21593, Abcam), anti‐CD68 (ab31630, Abcam), anti‐pp65 (3033, Cell Signaling Technology) and anti‐cleaved caspase 3 (9664s, Cell Signaling Technology) were used in the analysis. All immunohistochemistry signals were quantified by ImageJ software v1.60 (NIH, Bethesda, MD, USA).
Ab21593
Manufactured by Abcam
Sourced in United States
Ab21593 is a primary antibody product manufactured by Abcam. It is designed for use in various immunoassay applications. The product details and technical specifications are available on the Abcam website.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti p p65, by Cell Signaling Technology (1 mentions)
Anti cd90, by BioLegend (1 mentions)
Ab52915, by Abcam (1 mentions)
Ab25124, by Abcam (1 mentions)
Ab51072, by Abcam (1 mentions)
Ab9324, by Abcam (1 mentions)
Ab31630, by Abcam (1 mentions)
Sc 1745, by Santa Cruz Biotechnology (1 mentions)
Streptavidin hrp, by Vector Laboratories (1 mentions)
Ab166898, by Abcam (1 mentions)
B 1305 2, by Vector Laboratories (1 mentions)
Ab115957, by Abcam (1 mentions)
B 1235, by Vector Laboratories (1 mentions)
Rneasy plus tissue mini kit, by Qiagen (1 mentions)
Fl 1231, by Vector Laboratories (1 mentions)
Sc 32233, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab21593
1
Immunohistochemical Analysis of Mouse Joints
2
Glycoprotein Analysis in Pancreatic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
These experiments were performed as we previously described21 (link)–23 (link). For Western blot, antibodies for GAPDH (sc-32233; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Cosmc (sc-67480; Santa Cruz Biotechnology), CEL (ab79131; Abcam, Cambridge, UK; sc-34883; Santa Cruz Biotechnology), DMBT1 (MAB59151; R&D Systems, Minneapolis, MN, USA), elastase (ab21593; Abcam) and trypsin (ab166898; Abcam) were used. Ten micrograms per milliliter of Vicia villosa lectin (VVA) reactive against O-GalNAc (Tn antigen) (B-1235; Vector Laboratories, Burlingame, CA, USA) complexed with 1 µg streptavidin-HRP (21126; Pierce, Thermo Fisher Scientific, Grand Island, NY, USA) was used. For immunohistochemistry, antibodies for Tn Antigen (MA180055; Thermo Fisher Scientific, Grand Island, NY, USA), STn Antigen (ab115957; Abcam), insulin (8138; CST, Beverly, MA, USA) and VVA-fluorescein (FL-1231; Vector Laboratories) were used at a dilution of 1:10023 (link). Ten micrograms per milliliter of Sambucus nigra lectin (SNA) (B- 1305–2; Vector Laboratories, Burlingame, CA, USA) was complexed with 1 µg streptavidin-HRP. For real-time PCR, RNA was extracted with the RNeasy Plus Tissue Mini Kit (Qiagen).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!