Log In Sign up
The largest database of trusted experimental protocols

Nipbl

Manufactured by Fortis Life Sciences
Visit Supplier

NIPBL is a laboratory equipment product designed for scientific research. It serves as a tool for the analysis and study of genetic materials. The core function of NIPBL is to facilitate the investigation of chromatin structure and dynamics, which are important aspects of cellular biology and gene regulation.

Automatically generated - may contain errors

Lab products found in correlation

Smc1a, by Fortis Life Sciences (2 mentions) V9131, by Merck Group (1 mentions) Foxa1, by Santa Cruz Biotechnology (1 mentions) Fosl2, by Santa Cruz Biotechnology (1 mentions) Vinculin, by Merck Group (1 mentions) Hnf4a, by Santa Cruz Biotechnology (1 mentions) Rad21, by Abcam (1 mentions) H3k27ac, by Active Motif (1 mentions)

Spelling variants of this product

NIPBL antibody (2 citations)

2 protocols using nipbl

1

Lentiviral Knockdown of Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasmid catalog numbers and shRNA sequences are provided in Supplementary Table S3. Lentiviruses were produced as previously described58 (link). Cells were transduced with the lentiviruses for 24 h before puromycin selection for the indicated time. Validation of efficiency were performed by Western blot (Supplementary Figs 1 and 3) using MED1 (Bethyl A300-793A, 1:5000), SMC1A (Bethyl, A300-055A, 1:5000), NIPBL, (Bethyl, A301-779A, 1:1000), ERa (Santa Cruz, sc-543x, 1:5000), FOXA1 (Santa Cruz, sc-101058 1:1000), FOXA2 (Abnova, 89-019-034, 1:1000), HNF4a (Santa Cruz, sc-8987x, 1:1000), FOSL2 (Santa Cruz, sc-604x, 1:1000), GAPDH (Pierce, PIMA515738, 1:25000) and Vinculin (SIGMA, V9131, 1:50000).
Fournier M., Bourriquen G., Lamaze F.C., Côté M.C., Fournier É., Joly-Beauparlant C., Caron V., Gobeil S., Droit A, & Bilodeau S. (2016). FOXA and master transcription factors recruit Mediator and Cohesin to the core transcriptional regulatory circuitry of cancer cells. Scientific Reports, 6, 34962.
+ Open protocol
+ Expand
2

Antibody Characterization for Chromatin Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following are the antibodies used in this study: NIPBL (Bethyl Laboratories, A301-779A; Thermo Fisher Scientific, MA1-72534), YY1 (Active Motif, 61779), H3K27ac (Active Motif, 39133), GR (Santa Cruz Biotechnology, sc-393232; Thermo Fisher Scientific, MA1-510), SMC1a (Bethyl Laboratories, A300-055A; Abcam, ab133643), SMC3 (Abcam, ab9263), RAD21 (Abcam, ab992), H2B (Abcam, ab61250), and tubulin (Abcam, ab6160).
Rinaldi L., Fettweis G., Kim S., Garcia D.A., Fujiwara S., Johnson T.A., Tettey T.T., Ozbun L., Pegoraro G., Puglia M., Blagoev B., Upadhyaya A., Stavreva D.A, & Hager G.L. (2022). The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation. Science Advances, 8(13), eabj8360.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!