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Srebf1 primary antibody

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The SREBF1 primary antibody is a laboratory reagent used for the detection and analysis of the SREBF1 protein. SREBF1 is a transcription factor that plays a key role in the regulation of lipid and cholesterol metabolism.

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Lab products found in correlation

Ripa lysis buffer, by Thermo Fisher Scientific (4 mentions) Wet tank blotting, by Bio-Rad (2 mentions) Nupage gel, by Thermo Fisher Scientific (2 mentions) Irdye 680rd goat anti rabbit igg, by LI COR (2 mentions) Scd cd e10 antibody, by Abcam (2 mentions) Sm1 supplement, by STEMCELL (2 mentions) Irdye 800cw goat anti mouse igg, by LI COR (2 mentions) Odyssey detection system, by LI COR (2 mentions) Complete mini edta free protease inhibitor cocktail, by Roche (2 mentions) Gapdh d16h11 xp rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) β actin 8h10d10 mouse monoclonal antibody, by Cell Signaling Technology (1 mentions) Ab19862, by Abcam (1 mentions) Gapdh d16h11 xp rabbit mab, by Cell Signaling Technology (1 mentions) β actin 8h10d10 mouse mab, by Cell Signaling Technology (1 mentions)

2 protocols using srebf1 primary antibody

1

Western Blot Analysis of Lipid Metabolism Proteins

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Protein lysates were prepared in RIPA lysis buffer (ThermoFisher Scientific) with cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche). Western blot was performed using NuPAGE gel (ThermoFisher Scientific) with wet tank blotting (Bio-Rad) and Odyssey detection system (LI-COR). SREBF1 primary antibody (14088-1-AP, Proteintech), SCD (CD.E10) antibody (ab19862, Abcam), GAPDH (D16H11) XP rabbit monoclonal antibody (5174S, Cell Signaling), β-actin (8H10D10) mouse monoclonal antibody (3700S, Cell Signaling), and IRDye 800CW goat anti-mouse IgG (926-32210, LI-COR), IRDye 680RD goat anti-rabbit IgG (926-68071, LI-COR) secondary antibodies were used. Western blot was performed for cells cultured in different medium conditions. These include RPMI1640 with 10% FBS, with 10% delipidated FBS, with 10% human cerebrospinal fluid (991-19-P-5, Lee BioSolutions), or with 1% SM1 supplement (05711, STEMCELL Tech), or brain-slice-conditioned medium. Brain-slice-conditioned medium was prepared by submerging brain slices (150 μm) in RPMI1640 (no serum) for 48 h. Delipidated FBS was prepared as described51 (link).
Jin X., Demere Z., Nair K., Ali A., Ferraro G.B., Natoli T., Deik A., Petronio L., Tang A.A., Zhu C., Wang L., Rosenberg D., Mangena V., Roth J., Chung K., Jain R.K., Clish C.B., Vander Heiden M.G, & Golub T.R. (2020). A metastasis map of human cancer cell lines. Nature, 588(7837), 331-336.
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2

Western Blot Analysis of Lipid Metabolism Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were prepared in RIPA Lysis Buffer (ThermoFisher Scientific) + cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche). Western blot was performed using NuPAGE gel (ThermoFisher Scientific) + Wet/Tank Blotting (Bio-Rad) + Odyssey detection system (LI-COR). SREBF1 primary antibody (14088–1-AP, Proteintech), SCD (CD.E10) antibody (ab19862, Abcam), GAPDH (D16H11) XP® Rabbit mAb (5174S, Cell Signaling), β-Actin (8H10D10) Mouse mAb (3700S, Cell Signaling), and IRDye® 800CW Goat anti-Mouse IgG (926–32210, LI-COR), IRDye® 680RD Goat anti-Rabbit IgG (926–68071, LI-COR) secondary antibodies were used. Western blot was performed for cells cultured in different media conditions. These include RPMI1640 + 10% fetal bovine serum (FBS), or + 10% delipidated-FBS, or + 10% human cerebrospinal fluid (991–19-P-5, Lee BioSolutions), or + 1% SM1 supplement (05711, STEMCELL Tech), or brain slice conditioned media. Brain slice conditioned media was prepared by submerging brain slices (150 μm) in RPMI1640 (no serum) for 48 hours. Delipidated-FBS was prepared as described 51 (link).
Jin X., Demere Z., Nair K., Ali A., Ferraro G.B., Natoli T., Deik A., Petronio L., Tang A.A., Zhu C., Wang L., Rosenberg D., Mangena V., Roth J., Chung K., Jain R.K., Clish C.B., Vander Heiden M.G, & Golub T.R. (2020). A metastasis map of human cancer cell lines. Nature, 588(7837), 331-336.
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