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Rabbit anti klf4

Manufactured by Novus Biologicals
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Rabbit anti-KLF4 is a primary antibody that recognizes the Krüppel-like factor 4 (KLF4) protein. KLF4 is a transcription factor involved in the regulation of cellular processes. This antibody can be used for the detection of KLF4 in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Tcs sp8 x confocal microscope, by Leica camera (2 mentions) Vectashield dapi, by Vector Laboratories (2 mentions) Rabbit anti yy1, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit anti klf4

1

Immunofluorescence Analysis of Ramos Cells

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Rabbit anti-YY1 (Novus Biologicals NBP2-67391 1:250), Rabbit anti-KLF4 (Novus Biologicals NBP1-83940 1:250), anti-rabbit IgG Isotype control, AlexaFluor 488 Streptavidin (Jackson-lmmunoresearch, West Grove, CA, USA), and Vectashield- DAPI (Vector-laboratories, Burlingame, CA, USA) were used to stain Ramos cell lines transfected with INH or MIM-miR-7. Images were acquired using a Leica TCS SP8x Confocal Microscope (Wetzlar, Germany) and were analyzed with Leica software.
Morales-Martinez M., Vega G.G., Neri N., Nambo M.J., Alvarado I., Cuadra I., Duran-Padilla M.A., Huerta-Yepez S, & Vega M.I. (2020). MicroRNA-7 Regulates Migration and Chemoresistance in Non-Hodgkin Lymphoma Cells Through Regulation of KLF4 and YY1. Frontiers in Oncology, 10, 588893.
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2

Protein Expression in Ramos Cells

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Rabbit anti-YY1 (Cell Signaling, Tech), Rabbit anti-KLF4 (Novus Biologicals), anti-rabbit IgG, Isotype control, AlexaFluor 488 Streptavidin (Jackson-lmmunoresearch, West Grove, CA, USA) and Vectashield-DAPI (Vector-laboratories, Burlingame, CA, USA) were used to stain Ramos cell lines transfected with siRNA-YY1. Images were acquired using a Leica TCS SP8x Confocal Microscope (Wetzlar, Germany) and were analyzed with Leica software.
Morales-Martinez M., Valencia-Hipolito A., Vega G.G., Neri N., Nambo M.J., Alvarado I., Cuadra I., Duran-Padilla M.A., Martinez-Maza O., Huerta-Yepez S, & Vega M.I. (2019). Regulation of Krüppel-Like Factor 4 (KLF4) expression through the transcription factor Yin-Yang 1 (YY1) in non-Hodgkin B-cell lymphoma. Oncotarget, 10(22), 2173-2188.
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