All in vivo magnetic resonance experiments were carried out under isoflurane anaesthesia on a 9.4 T (400 MHz) MR system (Agilent Technologies) and using a quadrature-driven birdcage resonator (Rapid Biomedical) for high-resolution Magnetic Resonance cine Imaging (cine MRI) and cardiac 1H-MRS. Haemodynamic measurements in the left ventricle were made under isoflurane anaesthesia using a 1F-miro-tip catheter (Millar, Texas, USA) as previously described.12 (link) Dobutamine was infused at 16 ng/g BW/min via the jugular vein as a measure of contractile reserve. Mice were killed by cervical dislocation at the end of the experiments and their organs removed, washed in heparinised saline, blotted, and weighed.
9.4 t 400 mhz mr system
Manufactured by Agilent Technologies
Sourced in United States
The 9.4 T (400 MHz) MR system is a high-field magnetic resonance (MR) imaging system designed for advanced research applications. It provides a magnetic field strength of 9.4 tesla, enabling high-resolution imaging and spectroscopy. The system is capable of operating at a frequency of 400 MHz, which is suitable for a variety of MR-based studies.
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5 protocols using 9.4 t 400 mhz mr system
1
In Vivo MRI and Cardiac Assessment
2
Cardiac MRI Functional Imaging in Mice
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Mice were anaesthetized with isoflurane 1.5–2% in oxygen with ECG and respiration monitoring to ensure depth of anaesthesia and homeothermic temperature control. Imaging was carried out on a 9.4 T (400 MHz) MR system (Agilent Technologies) using a quadrature-driven birdcage resonator (Rapid Biomedical) as previously described.15 (link) Global cardiac functional parameters were measured from double-gated, multi-frame short-axis gradient-echo images covering the heart from the base to apex.
3
High-Field Preclinical MR Imaging
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MR imaging was carried out using a preclinical 9.4 T (400 MHz) MR system (Agilent, Santa Clara, US), comprising a horizontal magnet (inner diameter (i.d.) = 210 mm), an actively shielded gradient system (1 T/m, rise time 130 µs, i.d. = 60 mm) and a DirectDriveTM 2 console. A 13 mm i.d. quadrature-driven birdcage RF coil (Rapid Biomedical, Rimpar, Germany) was used to transmit and receive the MR-signals.
4
Non-Invasive Cardiac Creatine Quantification
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Myocardial creatine [Cr] was measured non-invasively on a 9.4 T (400 MHz) MR system (Agilent Technologies) using a quadrature-driven birdcage resonator (Rapid Biomedical). CrT- OE mice were anaesthetized with isoflurane and maintained at 1.5–2% in oxygen. Cardiac metabolite signals from a 2 µl voxel, placed in the interventricular septum, were acquired using a double-gated, double spin-echo sequence. Creatine signal was normalized to the water signal and total [Cr] estimated from the average creatine/water ratio using a calibration curve as reported previously [24] (link).
5
Cardiac Magnetic Resonance Imaging of Rat Hearts
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In vivo cine-MRI and subsequent ex vivo DT-MRI of rat hearts were acquired on a 9.4T (400 MHz) MR system (Agilent, Santa Clara, CA, USA). The cine-MRI dataset used a 2D multi-frame gradient echo sequence, and consisted of a stack of eight contiguous short-axis slices covering the heart from apex to the atrioventricular separation. Each short-axis slice comprised of 32–36 frames acquired throughout the cardiac cycle with an in-plane resolution of and a slice thickness of . A short-axis view in end-diastole is shown inFigure 1(c) . The DT-MRI images where subsequently acquired on excised rat hearts, fixed either in resting state or in contracture and embedded in agarose, at an isotropic resolution of as described in [18] (link).
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In vivo cine-MRI and subsequent ex vivo DT-MRI of rat hearts were acquired on a 9.4T (400 MHz) MR system (Agilent, Santa Clara, CA, USA). The cine-MRI dataset used a 2D multi-frame gradient echo sequence, and consisted of a stack of eight contiguous short-axis slices covering the heart from apex to the atrioventricular separation. Each short-axis slice comprised of 32–36 frames acquired throughout the cardiac cycle with an in-plane resolution of and a slice thickness of . A short-axis view in end-diastole is shown in
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