Hydroxylammonium chloride (reagent grade >98% w/w), ammonium acetate (>99% w/w), (S,S)-ethylenediamine-N,N′-disuccinic acid-trisodium salt solution (35% v/v), hydrogen peroxide solution (30% v/v), acetic acid (ACS reagent >97% v/v), and nitric acid (ACS reagent >67% v/v) from Sigma-Aldrich (Saint Louis, MI, USA) were used as reagents. Only ultra-pure water was used for analytical preparations and dilution. Atomic Absorption Spectrometer (AAS) standards were employed for Cd, Cr, Cu, Fe, Pb, Zn (Carlo Erba, Reagenti), Mn, and Ni (Fluka Reagents).
Hydroxylammonium chloride
Manufactured by Merck Group
Sourced in United States, Germany
Hydroxylammonium chloride is a chemical compound with the formula H3NO·HCl. It is a crystalline solid and is commonly used as a reducing agent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Merck Group (5 mentions)
Hydrogen peroxide, by Merck Group (4 mentions)
Ammonium acetate, by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Triton x 100, by Merck Group (2 mentions)
Iron 2 chloride tetrahydrate, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Carl Roth (2 mentions)
Spectrapor 6, by Carl Roth (2 mentions)
Iron 3 chloride hexahydrate, by Carl Roth (2 mentions)
Ringer s solution, by Baxter (2 mentions)
Diic1 5, by Thermo Fisher Scientific (2 mentions)
Ammonium chloride, by Carl Roth (2 mentions)
Formic acid, by Carl Roth (2 mentions)
Nitric acid, by Merck Group (1 mentions)
Hydrogen peroxide solution, by Merck Group (1 mentions)
Acs reagent, by Merck Group (1 mentions)
Reagenti, by Carlo Erba (1 mentions)
Hydrogen peroxide h2o2, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
9 protocols using hydroxylammonium chloride
1
Trace Metal Analysis in Samples
2
Assessing Heavy Metal Levels in Brahmaputra River Sediments
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Ultra-pure water was used throughout the dilution procedure, and all reagents were analytical grade unless otherwise stated. Acetic acid (glacial, 100% supra pure, Sigma Aldrich), hydroxylammonium chloride (99.999% trace metal basis, Sigma Aldrich), hydrogen peroxide (30%, stabilized), ammonium acetate (99.999% trace metal basis, Sigma Aldrich) and nitric acid (60%, Suprapur Merck) were used. All glassware was cleaned with nitric acid and rinsed with deionized water before use.
To assess the accuracy of the method, a certified reference material (CRM, BCR 701) was subjected to the BCR protocol. It was observed that the measured values of BCR 701 fractions are in good agreement with their certified value (refer toSI, Table S2 ). Borah et al. [8 (link)] estimated the average total concentration of Cu (40.9 mg/kg) and Zn (51.8 mg/kg) for the Brahmaputra River bed sediment flowing in Assam state that is in good range with the total concentrations obtained in this study as Cu (46.88 mg/kg) and Zn (38.74 mg/kg).
To assess the accuracy of the method, a certified reference material (CRM, BCR 701) was subjected to the BCR protocol. It was observed that the measured values of BCR 701 fractions are in good agreement with their certified value (refer to
3
Enzymatic Hydrolysis of Seal Meat
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Fresh adult harp seal meat (Pagophilus groenlandicus) loins were obtained from SeaDNA Canada Inc., Québec, Canada. Antioxidant hydrolysates from seal meat were prepared using enzymatic hydrolysis in phosphate buffer (pH 7.5) following an established protocol [13 (link)]. Ethylenediaminetetraacetic acid (EDTA), ethanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), iron sulfate (FeSO4), 1,10-phenanthroline, sodium phosphate, hydroxylammonium chloride, and hydrogen peroxide (H2O2) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
4
Characterization of PIFA and Gasification Slag
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The PIFA used in this study was acquired from a hazardous waste incinerator in southern China. The gasification slags used in this study were acquired from a refinery in Shandong Province, China. The PIFA and gasification slag samples were crushed and sieved to a size <.125 mm using a ball grinding mill (FRITSCH, Pulverisette 7, Germany). The collected samples were dried in an oven at 105°C for 24 h before the analyses. The chemical compositions of the samples were determined using XRF (ZSX Priums, RIGAKU, Japan), and the results are presented in Table 1 . Acetic acid, hydroxylammonium chloride, ammonium acetate and hydrochloric acid used in this study were purchased from Sigma-Aldrich (Shanghai, China) and were used as received.
5
Enzyme PEGylation and Characterization
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Horse heart cytochrome c (Cyt-c, ≥95% purity), 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) (ABTS, ≥95% purity), hydrogen peroxide (99% purity) and hydroxylammonium chloride (99% purity) were obtained from Sigma-Aldrich® (St. Louis, MO, USA). Methoxy PEG N-hydroxysuccinimide ester (mPEG-NHS, 5 kDa) with high purity was acquired from Nanocs® (New York, NY, USA). The aqueous buffer used in the PEGylation reaction was potassium phosphate buffer (100 mM). All other reagents were of analytical grade. The water used was double-distilled, passed through a reverse osmosis system and was further treated with Direct-Q® 8 UV remote water purification system (Merck®, São Paulo, Brazil).
6
Chitosan-Based Nanoparticle Fabrication
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Low molecular weight chitosan (CS), sodium tripolyphosphate (TPP) penta basic, dimethyl sulfoxide (DMSO), 3,3′,5,5′-tetramethylbenzidine (TMB), sodium citrate dihydrate, hydrogen tetrachloroaurate (III) tetrahydrate (HAuCl4·4H2O), hydrogen peroxide (H2O2), hydroxylammonium chloride (NH2OH·HCl), sodium carbonate anhydrous (Na2CO3), sodium hydrogen carbonate (NaHCO3), disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), sodium dihydrogenphosphate dihydrate (NaH2PO4·2H2O), acetic acid (HAc), and sodium acetate (NaAc) were all purchased from Merck. All reagents were of analytical grade and used without any further purification. Ninhydrin, hydrindatin were purchased from Ingos (Prague, Czech Republic). Aqueous solutions for size analysis were prepared using PURELAB® Ultra (Elga, High Wycombe, United Kingdom) resistivity 18 mΩ-cm. For other purposes deionized water was used.
7
Synthesis and Characterization of Iron Nanoparticles
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Iron(II) chloride tetrahydrate (FeCl2⋅4H2O), hydroxylammonium chloride, bovine serum albumin (BSA), and Eppendorf ultrafiltration tubes with a molecular weight cutoff (MWCO) of 3 kDa were purchased from Merck (Darmstadt, Germany). Iron(III) chloride hexahydrate (FeCl3⋅6H2O), dialysis tubes (Spectrapor 6, MWCO 8 kDa), ammonium chloride, formic acid, hydrochloric acid 25 %, ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), sodium thiosulfate, silver nitrate, sodium carbonate, and ammonia solution 25 % were supplied by Roth (Karlsruhe, Germany). Propidium iodide (PI), sodium citrate, triton X-100, ninhydrin, MES, HEPES, and poly(acrylic acid-co-maleic acid) solution (Mw = 3000 Da) were purchased from Sigma-Aldrich (St Louis, MO, USA). Ringer’s solution was bought from Baxter Healthcare (Zurich, Switzerland). Annexin V-FITC (AxV), Hoechst 33342 (Hoe), and DiIC1(5) (hexamethylindodicarbocyanine iodide dye [DiI]) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
8
Gold(III) Chloride Immunoassay Protocol
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Gold(III)
chloride solution
(HAuCl4, 99.99% trace metals basis), bovine serum albumin
(BSA, ≥98%), 3,3′,5,5′-tetramethylbenzidine (TMB
substrate, ≥99%), acetic acid (glacial, ≥99.7%), Tween-20
(≥40.0%), brain heart infusion broth (BHI broth), and agar
were purchased from Sigma-Aldrich Co., Ltd., USA. Trisodium citrate
dihydrate (HOC(COONa)(CH2COONa)2·2H2O, ≥99%), hydroxylammonium chloride (NH2OH·HCl, ≥99%), hydrogen peroxide (H2O2, 30%), sodium acetate trihydrate (CH3COONa·3H2O, ≥99%), sodium chloride (NaCl, ≥99), potassium
chloride (KCl, ≥99), disodium hydrogen phosphate (Na2HPO4, ≥99%), and potassium dihydrogen phosphate
(KH2PO4, ≥99.5%) were purchased from
Merck, USA. K. pneumoniae polyclonal
antibody (Rabbit, IgG) was purchased from Invitrogen, USA. Immunoreactions
were performed in Pierce 8-Well Polystyrene Strip Plates, Corner Notch
(Thermo Scientific, Inc., USA).
chloride solution
(HAuCl4, 99.99% trace metals basis), bovine serum albumin
(BSA, ≥98%), 3,3′,5,5′-tetramethylbenzidine (TMB
substrate, ≥99%), acetic acid (glacial, ≥99.7%), Tween-20
(≥40.0%), brain heart infusion broth (BHI broth), and agar
were purchased from Sigma-Aldrich Co., Ltd., USA. Trisodium citrate
dihydrate (HOC(COONa)(CH2COONa)2·2H2O, ≥99%), hydroxylammonium chloride (NH2OH·HCl, ≥99%), hydrogen peroxide (H2O2, 30%), sodium acetate trihydrate (CH3COONa·3H2O, ≥99%), sodium chloride (NaCl, ≥99), potassium
chloride (KCl, ≥99), disodium hydrogen phosphate (Na2HPO4, ≥99%), and potassium dihydrogen phosphate
(KH2PO4, ≥99.5%) were purchased from
Merck, USA. K. pneumoniae polyclonal
antibody (Rabbit, IgG) was purchased from Invitrogen, USA. Immunoreactions
were performed in Pierce 8-Well Polystyrene Strip Plates, Corner Notch
(Thermo Scientific, Inc., USA).
9
Fluorescent Labeling of Annexin A5
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Iron (II) chloride tetrahydrate (FeCl2·4H2O), hydroxylammonium chloride, bovine serum albumin (BSA), and Eppendorf ultrafiltration tubes with a molecular weight cutoff (MWCO) of 3 kDa were purchased from Merck (Darmstadt, Germany). Iron (III) chloride hexahydrate (FeCl3·6H2O), dialysis tubes (Spectrapor 6, MWCO 8 kDa), ammonium chloride, formic acid, hydrochloric acid 25%, and ammonia solution 25% were supplied by Roth (Karlsruhe, Germany). Propidium iodide (PI), sodium citrate, triton X-100, ammonium formate, lauric acid, and acetone were purchased from Sigma-Aldrich (St Louis, MO, USA). Sulfosalycylic acid solution 20% was bought from Applichem (Darmstadt, Germany). Mitoxantrone (MTO) solution (2 mg/mL) was purchased from TEVA Pharma (Ulm, Germany). Ringer’s solution was bought from Baxter Healthcare (Zurich, Switzerland). Falcon ultrafiltration tubes (MWCO 100 kDa) were purchased from Sartorius (Goettingen, Germany). Recombinant chicken Annexin A5 (AxA5; responsif GmbH, Erlangen, Germany) was labeled with fluorescein isothiocyanate (AxA5-FITC) according to the manufacturer’s instructions (Sigma-Aldrich). Hoechst 33342 (Hoe) and DiIC1(5) (hexamethylindodicarbocyanine iodide dye [DiI]) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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