Tet2fl fl mice

Generation of Vav-TRAF6 mice was described in our previous study^{5 (link)}. To distinguish CD45.2⁺ cells based on YFP expression, Vav-TRAF6 mice were crossed to mice expressing a Cdc42-YFP transgene that results in ubiquitous YFP expression. Tet2^fl/fl mice (Jackson Laboratory, 017573) and Traf6^fl/fl mice^{14 (link)} were crossed with VavCre mice (Jackson Laboratory, 008610). A20^fl/fl mice were kindly provided by A. Ma and described elsewhere^{49 (link)}. For conditional deletion of A20, A20^fl/fl mice were crossed with RosaCreER mice. UBC-GFP mice (Jackson Laboratory, 004353) were purchased from the Jackson Laboratory. NF-kB-GFP reporter mice were generously provided by C. Jobin^{29 (link)}. All mice were bred, housed and handled in the Association for Assessment and Accreditation of Laboratory Animal Care-accredited animal facility of Cincinnati Childrens Hospital Medical Center.

All animals were maintained on a pure C57BL/6 genetic background and housed under Specific Pathogen Free (SPF) at Washington University in Saint Louis with a 12-hour dark/light cycle, 22–25 °C, and 50–60% humidity with water and food provided ad libitum. Tet2^−/− and Tet2^fl/fl mice were obtained from The Jackson Laboratory (Stock No:023359 and 017573)^{59 ,60} . Rorc^Cre mice were provided by A. Tumanov⁶¹ . Il7r^iCre mice were obtained from HR Rodewald⁶² . Mice used for experiments were between 8–12 weeks of age and were on a C57BL/6 background. Control mice were littermates. All studies performed on mice were done in accordance with the Institutional Animal Care and Use Committee at Washington University in St. Louis which approved all protocols used in this study.