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4 protocols using hexane

1

Synthesis of Nanoparticles using Oleylamine

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Oleylamine (OAm, >70%) and hexane (99.5%), were both purchased from Tokyo Chemical Industry Co. Ltd., Shanghai. Pt(acac)2 (99.99%), Nafion (5% w/w solution) were all obtained from Alfa Aesar. Ammonium fluoride (≥96%), Ammonium chloride (≥99.5%), Ammonium bromide (≥99%), chloroform (≥99%) and ethanol (≥99.5%) were all purchased from Sinopharm Chemical Reagent Co. Ltd. All the chemicals were of analytical grade and used as received without further purification.
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2

Synthesis and Characterization of Magnetic Nanoparticles

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Iron acetylacetonate (Fe(acac)3), cobalt acetylacetonate (Co(acac)2), phenyl ether, benzyl ether, oleic acid (OA), and absolute ethanol were obtained from (ACROS ORGANIC, Morris Plains, NJ, USA), Tungsten hexachloride (WCl6) and 1-octadecene were purchased from (Alfa Aesar Inc., Haverhill, MA, USA), Oleylamine (OAm), hexane, ethylene glycol, 3-(3,4-dihydroxy)hydrocinnamic acid (DHCA), tetrahydrofuran (THF), NaOH, and phosphate buffered saline (PBS, concentration of PO42− ions (0.0067 M) were acquired from Tokyo Chemical Industry (TCI Co, Tokyo, Japan), (Fisher chemicals, Pittsburgh, PA, USA), (J.T.Baker, Phillipsburg, NJ, USA), (Sigma Aldrich, St. Louis, MO, USA) (ECHO CHEMICAL CO., LTD., Taipei, Taiwan), (SHOWA Chemical, Tokyo, Japan), and HyCloneTM, Marlborough, MA, USA) respectively. The above chemicals were used without further purification. The 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco’s Phosphate Buffered Saline (DPBS) were purchased from (Sigma Aldrich, St. Louis, MO, USA).
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3

Solvent Purification and TLC Monitoring

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All chemical reagents were commercially available.
The solvents (chloroform (99%), dimethyl sulfoxide (DMSO) (99%), ethyl
acetate (99%), hexane (99%), diethyl ether (99%), tetrahydrofuran
(THF) (99%), and toluene (99%)) were purchased from Tokyo Chemical
Industry Co., Ltd. and were directly used without further purification.
Unless otherwise stated, the reactions were monitored by thin layer
chromatography on silica gel plates (60 F254) and visualized with
ultraviolet light or iodine spray. Column chromatography was performed
on silica gel (60–120 mesh) using hexane and ethyl acetate.
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4

Synthesis and Characterization of Novel Compounds

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Hoechst 33342 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Invitrogen (MA, USA). Dimethyl sulfoxide (DMSO) and epidermal growth factor (EGF) were purchased from Sigma-Aldrich (MI, USA). Chloroform (CHCl3), deuterated Chloroform (CDCl3), dichloromethane (CH2Cl2), Dimethyl sulfoxide, ethyl acetate (CH3COOC2H5), hexane (C6H14), isotonicotinoyl chloride hydrochloride (C6H4ClNO), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (C8H17N3·HCl) (ECDI), dichloromethane (CH2Cl2) and silica gel 7734 were purchased from Tokyo Chemical Industry (Tokyo, Japan).
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