Koptec 200 proof ethanol

For studying kit buffer inhibitors, LAMP and qPCR reactions were spiked to 5 × 10⁴ cp/rxn λ phage DNA (NEB) and supplemented with half-log dilutions of either Koptec 200-proof ethanol (V1001, Decon Labs, King of Prussia, PA, USA), Viral RNA Wash Buffer 1x (R1034-2-48, Zymo Research, Tustin, CA, USA), Buffer PE (19065, Qiagen, Germantown, MD, USA), Zymo DNA/RNA Shield 1x (R1200-125), Zymo Viral DNA/RNA Buffer (D7020-1-100), or Qiagen Buffer PB (19066) to the appropriate final concentration. For selecting the optimal TPW, LAMP and qPCR reactions were spiked with 1 µL of 5 × 10⁴ cp/µL λ phage DNA, diluted to 10 µL, and an additional 1 µL was added of either nuclease-free water, 200 proof ethanol, isopropanol (BP2618-500, Thermo Fisher Scientific, Waltham, MA, USA), 1-butanol (3000-04, Mallinckrodt Chemicals), isopentanol (2992-04, Mallinckrodt Chemicals), 1-hexanol (H13303–100 mL, MilliporeSigma, St. Louis, MO, USA), 1-heptanol (H2805-250 mL, MilliporeSigma), 1-octanol (SHBH2844V, MilliporeSigma), 1-nonanol (131210–100 mL, MilliporeSigma), 1-decanol (2397563–50 g, MilliporeSigma), 1-undecanol (MKCG3271, MilliporeSigma), 2-dodecanol (D221503-5G, MilliporeSigma), 5 cSt silicone oil (317667-250 mL, MilliporeSigma), or Fluorinert FC-40 (ZF-0002-1308-0, 3 M, St. Paul, MN, USA).