Data collection of negative stain grids was performed on a Tecnai LaB6 G2 Spirit transmission electron microscope (FEI) operating at 120 keV (EM STP, The Francis Crick institute). Micrographs were collected using a 2Kx2K GATAN Ultrascan 100 camera at a nominal magnification of 30,000 (3.45 Å pixel size) or 21,000 (4.92 Å pixel size, train classes) within a -0.5 to 2.5 μm defocus range. Analysis of positive stain grids was performed on a Tecnai G2 F20 TWIN electron microscope operating at 200 keV (FEI; Electron Microscopy Centre, Imperial College London) equipped with a Falcon II direct electron detector (FEI). Micrographs were collected at a nominal magnification of 50,000 (2.05 Å pixel size) in a defocus range from -3 to -6 μm.
Tecnai g2 f20 twin electron microscope
Manufactured by Thermo Fisher Scientific
The Tecnai G2 F20 TWIN electron microscope is a high-performance transmission electron microscope (TEM) designed for advanced materials analysis and nanoscale imaging. It features a field emission gun (FEG) source, providing high-resolution imaging and analysis capabilities. The Tecnai G2 F20 TWIN is capable of performing a range of analytical techniques, including electron diffraction, energy-dispersive X-ray spectroscopy (EDS), and electron energy-loss spectroscopy (EELS).
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2 protocols using tecnai g2 f20 twin electron microscope
1
Negative and Positive Stain TEM Imaging
2
Visualizing MinE1-31 Peptide Structures
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MinE1-31 peptide (4 μl of 6 μM) prepared in three imaging buffers was spotted onto a Ultrathin Carbon Type A grid (Pelco®) and incubated for 1 min at room temperature. Prior to use, the grid was glow-discharged by the EMS 100 glow discharge unit for 15 seconds. The sample was then stained with 4% uranyl acetate for 70 sec followed by air drying. Transmission electron microscopy (TEM) images were collected using a Tecnai G2 F20 TWIN electron microscope with a field emission gun (FEI company) at 200 KeV, which was equipped with a Gatan UltraScan 4000 CCD Camera (4,096 × 4,096; 15-μm pixel size), and processed using Digital Micrograph software (Version 3.9.5, Gatan).
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