Rnf20

Manufactured by Proteintech

Sourced in China

RNF20 is a recombinant protein produced by Proteintech. It functions as an E3 ubiquitin-protein ligase that catalyzes the monoubiquitination of histone H2B, which is involved in the regulation of gene expression.

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Lab products found in correlation

Nestin, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Proteintech (1 mentions) Alexa conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Trimethyl histone h3 lys27, by Merck Group (1 mentions) H3k9me3, by Merck Group (1 mentions) Acsbg1, by Abcam (1 mentions) Glast, by Proteintech (1 mentions) Ubiquityl histone h2b lys 120, by Cell Signaling Technology (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) H4k16ac, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β actin, by Proteintech (1 mentions) Nf κb2, by Proteintech (1 mentions) Kdm5a, by Proteintech (1 mentions) Jmjd6, by Proteintech (1 mentions) Kdm4a, by Novus Biologicals (1 mentions) Kdm4c, by Novus Biologicals (1 mentions)

2 protocols using rnf20

Multiparametric Immunostaining and Western Blotting

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The primary antibodies used in immunostaining were as follows: RNF20 (21615-1-AP; Proteintech, Rosemont, IL, USA), NESTIN (MAB353; Millipore, Darmstadt, Germany), ACSBG1 (ab118154; Abcam, Cambridge, UK), GFAP (G6171; Sigma, St. Louis, MO, USA), GFAP (Z0334 Dako, Santa Clara, CA, USA), GFAP (60190-1-Ig; Proteintech), GLAST (BMP009; MBL, Beijing, China), Tenascin C (110-68136; NB, Littleton, CO, USA), Ki67 (ab137876; Abcam), FLAG (F1804; Sigma), STAT3 (#9139; Cell Signaling Technology, Beverly, MA, USA). The primary antibodies used in western blotting were as follows: RNF20 (21615-1-AP; Proteintech), GLAST (20785-1-AP; Proteintech), ACSBG1 (ab118154; Abcam), GFAP (G6171; Sigma), STAT3 (#9139; Cell Signaling Technology), phospho-STAT3 (#9145; Cell Signaling Technology), Ubiquityl-Histone H2B (Lys 120) (#5546; Cell Signaling Technology), H4K16ac (07-329; Millipore), H3K9me3 (07-442; Millipore), Trimethyl-Histone H3 (Lys 27) (07-449; Millipore), H2B (ab1790; Abcam), FLAG (F1804; Sigma), HA (M20003; Abmart, Shanghai, China), β-ACTIN (20536-1-AP; Proteintech). Alexa-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) were used for immunostaining. IR Dye (LI-COR Biosciences, Lincoln, Nebraska, USA) 800 CW donkey anti-rabbit and 680 CW donkey anti-mouse secondary antibodies were used in western blotting.

Liang Q., Xia W., Li W, & Jiao J. (2017). RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain. Cell Death and Differentiation, 25(2), 294-306.

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Antibody Sources for Protein Studies

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Specific antisera were obtained from the following sources: HIV-1 Tat (ab42359 & ab43014), GFP (sc-9996), HA (Sigma, H9658-.2ML, clone HA-7 & Novus, NB600363), Flag (ThermoSientific, MA1-91878-1MG, clone FG4R), RNAPII-CTD (sc-56767, clone 8WG16), RNAPII CTD-Ser2P (Bethyl, A300-654A), RNAPII CTD-Ser5P (Active Motif, 39749), RNAPII CTD-Ser7P (Millipore, 04–1570), NF-κB/p65 (sc-372), NF-κB2 (ProteinTech, 10409-2-AP), H2B (Millipore, 07–371), H3 (Millipore, 05–928), TBP (sc-273), TAF4 (sc-136093), Cyclin T1 (sc-10750), CDK9 (sc-8338), RNF20 (ProteinTech, 21625-1-AP), KDM4A (Novus, NB110-40585), KDM4B (Novus, NB100-74605), KDM4C (NBP1-49600), KDM4D (NBP1-03357), KDM5A (NB110-40499), KDM5B (NB100-97821), JMJD6 (NBP1-71693), NELF-A (sc-32911), Csn3 (sc-100693), Csn8 (sc-393482), PRMT6 (ProteinTech, 15395-1-AP), SHMT1 (ProteinTech, 14149-1-AP), SHMT2 (ProteinTech, 11099-1-AP), BRCC36 (Novus, NBP1-76831), Ubquitin (Millipore, 05–944), K48Ub (Millipore, 05–1307), K63Ub (Millipore, 05–1313), p62 (ProteinTech, 18420-1-AP), Rabbit IgG (sc-2027), Mouse IgG (sc-2025) and HRP-conjugated 2nd antibodies (sc-2004, sc-2005 & sc-2033).

Xu M., Moresco J.J., Chang M., Mukim A., Smith D., Diedrich J.K., Yates JR I.I.I, & Jones K.A. (2018). SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy. PLoS Pathogens, 14(5), e1007071.

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