Collagen coated dishes

Manufactured by Corning

Sourced in United States

Collagen-coated dishes are a type of laboratory equipment designed for cell culture applications. The dishes are coated with a layer of collagen, a naturally occurring protein that serves as a substrate for cell attachment and growth. This product provides a supportive and physiologically relevant environment for the culture of various cell types.

Automatically generated - may contain errors

Lab products found in correlation

11 protocols using collagen coated dishes

Differentiating PC-12 Cells into Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat pheochromocytoma cells (PC-12 cells) were obtained from American Type Culture Collection (ATCC). The cells were grown in suspension in RPMI-1640 (ATCC) with 10% heat inactivated horse serum, 5% fetal bovine serum (Gibco) at 37°C and 5% CO₂. To differentiate the cells into neuronal cells PC-12 cells were plated in collagen coated dishes (Corning) and were grown in RPMI-1640 with NGF (100ng/ml, Alomone Labs) and 1% Horse Inactivated serum (Gibco). The cells were grown for 10 days and the medium was changed every 2 days. Neuronal morphology and robust neurite outgrowth was confirmed by microscopy. NGF was removed overnight before the day of experiment. PC-12 cells were treated with CEPO 100ng/ml for 3hrs. Vehicle-treated (PBS) cells were used as control.

Tiwari N.K., Sathyanesan M., Schweinle W, & Newton S.S. (2018). Carbamoylated erythropoietin induces a neurotrophic gene profile in neuronal cells. Progress in neuro-psychopharmacology & biological psychiatry, 88, 132-141.

+ Open protocol

+ Expand

Cell Culture Protocols for HeLa, SH-SY5Y, and Flp-In-293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells were obtained from RIKEN BRC, which participates in the National Bio-Resource Project of the MEXT, Japan. HeLa cells were maintained in RPMI 1640 medium (Nacalai Tesque) with 10% heat-inactivated fetal bovine serum (FBS) (Sigma) and 1% antibiotic–antimycotic solution (Gibco, Gaithersburg, MD, USA) at 37 °C and 5% CO₂.
SH-SY5Y cells (DS Pharma Biomedical, Osaka, Japan) were seeded onto collagen-coated dishes (Corning, Cambridge, MA, USA) and maintained in Dulbecco’s modified Eagle’s medium-F12 (1:1) medium (Nacalai Tesque) supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin (Gibco) at 37 °C and 5% CO₂.
Flp-In-293 cells (Thermo Fisher Scientific, Waltham, MA, USA) were seeded onto collagen-coated dishes and maintained in Dulbecco’s modified Eagle’s medium (Nacalai Tesque) supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin at 37 °C and 5% CO₂.

Watanabe K., Nawachi T., Okutani R, & Ohtsuki T. (2021). Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer. Scientific Reports, 11, 14936.

+ Open protocol

+ Expand

Proteome Analysis of Differentiated PC-12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat pheochromocytoma cells (PC-12 cells) were obtained from the American Type Culture Collection (ATCC). The cells were grown and cultured as mentioned previously with some modifications [16 ]. The cells were grown in suspension in RPMI-1640 (ATCC) with 10% heat-inactivated horse serum and 5% fetal bovine serum (Gibco, Gaithersburg, MD, USA) at 37 °C and 5% CO₂. To differentiate the cells into neuronal cells, PC-12 cells were plated in collagen-coated dishes (Corning, Corning, NY, USA) and were grown in RPMI-1640 with NGF (100 ng/mL, Alomone Labs, Jerusalem, Israel) and 1% horse inactivated serum (Gibco). The cells were grown for 10 days and the medium was changed every 2 days. Neuronal morphology and robust neurite outgrowth were confirmed by microscopy. Nerve growth factor (NGF) was removed overnight before the day of experiment. PC-12 cells were treated with EPO and CEPO 100 ng/mL for 5 h. Vehicle-treated (PBS) cells were used as controls. Four replicates were used for each control, and CEPO- and EPO-treated samples were used for label-free quantitative proteome analysis.

Tiwari N.K., Sathyanesan M., Kumar V, & Newton S.S. (2021). A Comparative Analysis of Erythropoietin and Carbamoylated Erythropoietin Proteome Profiles. Life, 11(4), 359.

+ Open protocol

+ Expand

Myoblast Culture and Differentiation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For myoblast culture, muscle stem cells were purified by fluorescence-activated cell sorting (FACS) as described earlier²⁸ (link) and plated on collagen-coated dishes (Corning) in Ham’s F10 medium (Thermo Scientific) supplemented with 20% fetal bovine serum (FBS) (Thermo Scientific) and 5 ng/mL basic fibroblast growth factor (FGF) (Thermo Scientific). Human myoblasts were cultured as previously described.⁴⁰ (link) Differentiation was carried out in DMEM supplemented with 5% horse serum (HS).
C26 and LL/2 cells were cultured in growth media (DMEM, 10% FBS) in a tissue culture incubator at 37°C, 5% CO₂, and 95% humidity. C26 and LL/2 cells were cultured in differentiation media of primary myoblasts for approximately 3 days. Media were filtered with a 0.2-μm syringe filter, and 80% of total volume of primary myotube differentiation media was replaced with C26 or LL/2 media at day 3 of the differentiation assay. In addition, recombinant Wnt7a (50 μg/mL; R&D Systems, #3008-WN-025), recombinant IGF-1 (50 μg/mL, Sigma-Aldrich, #I1271-.1MG), or rapamycin (20 μg/mL, Cell Signaling, #9904) was added to the cells.

Schmidt M., Poser C, & von Maltzahn J. (2020). Wnt7a Counteracts Cancer Cachexia. Molecular Therapy Oncolytics, 16, 134-146.

+ Open protocol

+ Expand

Stimulation of Immune Cells and Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK 293 cells were grown in DMEM supplemented with 10% FCS, L-glutamine, penicillin/streptomycin, sodium pyruvate, and HEPES. U937 and THP1 cell lines were grown in RPMI supplemented as above, and differentiated prior to stimulation using 100 nM phorbol myristoyl acetate (PMA) for 24 hours and then rested in media lacking PMA for 24 hours. Single-donor primary human hepatocytes (Lonza, catalog #: HUCPG; donor #s: HUM17299A, HUM173531) were thawed and plated as recommended on collagen-coated dishes (Corning) at 0.5 million/well in 24 well format.
HEK 293 cells were plated at 0.5 million/well in a 6 well dish in 2 mL media the day before stimulation for protein harvest. For RNA harvest and qPCR, U937 cells were plated at 0.25 million/well in a 24 well dish. In the 6 well dish format, cells received 8 μg of CT DNA, ISD100, or pcDNA3 complexed with 8 μl of Lipofectamine 2000. 10 μM cGAMP was complexed with 8 μl Lipofectamine, and 1 μg RIG-I ligand was complexed with 1 μl Lipofectamine to achieve comparable induction of IFN across treatments in competent cells. Stimulations done in 24 well plates were scaled by ¼. Etoposide (prepared in DMSO) was diluted in culture media to 50 μM, and untreated cells received the same volume of DMSO. Cells were irradiated with 30 Gy using a Rad Source RS 2000 X-irradiator.

Burleigh K., Maltbaek J.H., Cambier S., Green R., Gale M., James R.C, & Stetson D.B. (2020). Human DNA-PK activates a STING-independent DNA sensing pathway. Science immunology, 5(43), eaba4219.

+ Open protocol

+ Expand

Adipocyte Differentiation from Mouse iWAT

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male C57BL/6J mice (7–9 weeks of age) were obtained from Japan SLC (Shizuoka, Japan) and housed in an animal room (23 ± 3°C, 12 h light:dark cycle, lights on from 8:00 to 20:00 h) and allowed ad libitum access to water and a standard laboratory diet (CE-2, CLEA Japan, Tokyo, Japan) [21 (link)]. After 1 week of breeding, mice were killed and iWAT was carefully removed and the SVF was isolated [22 ]. Briefly, isolated iWAT was minced and suspended and digested in 0.2% collagenase (125 collagen digestion units/mL, C6885, Sigma-Aldrich Japan, Tokyo, Japan) solution at 37°C for 30 min. After digestion, the cell suspension was filtered using a cell strainer and centrifuged. The obtained pellet (SVF) was re-suspended in DMEM supplemented with 10% FBS, and plated on collagen-coated dishes (CORNING, NY). Confluent cells were differentiated and induced to adipocytes as well as C3H10T1/2 cells. The experimental design was approved by the Animal Experiment Committee, Chubu University, and the mice were maintained in accordance with their guidelines (Permission No. 2610031 and 2710011).

Nishikawa S., Aoyama H., Kamiya M., Higuchi J., Kato A., Soga M., Kawai T., Yoshimura K., Kumazawa S, & Tsuda T. (2016). Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. PLoS ONE, 11(9), e0162512.

+ Open protocol

+ Expand

Isolation and Culture of PDAC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

After explantation, tumor tissue was stored in RPMI (Life Technologies) without any supplements on ice. The tumor tissue was then minced on ice into 1-2 mm³ cubes. The minced tissue was then transferred into digestion media (RPMI containing 5 mg/mL Collagenase II (Life Technologies) and 1.25 mg/mL dispase (Life Technologies)) and incubated at 37°C with agitation to dissociate the tumor tissue. Cell suspension was subsequently filtered through a 100 μm mesh and cells were collected by centrifugation (300 xg, 5 min) at room temperature. The cell pellet was resuspended in growth media consisting of 50% Keratinocyte-SFM (Life Technologies) supplemented with Epidermal Growth Factor and Bovine Pituitary Extract (Life Technologies), 2% FBS (Life Technologies), and 1% antibiotic-antimycotic (Life Technologies) and 50% advanced DMEM F12 (Life Technologies) supplemented with 10% FBS (Life Technologies), 10 mM HEPES (Life Technologies), 1% Glutamax (Life Technologies), 1% antibiotic-antimycotic (Life Technologies). Cells were cultured on collagen-coated dishes (Corning) in a humidified incubator at 37°C with 5% CO₂. To obtain pure PDAC cell lines, cells were treated with differential trypsinization until no contaminating cells were detected by visual inspection under the microscope.

Dorsch M., Kowalczyk M., Planque M., Heilmann G., Urban S., Dujardin P., Forster J., Ueffing K., Nothdurft S., Oeck S., Paul A., Liffers S.T., Kaschani F., Kaiser M., Schramm A., Siveke J.T., Winslow M.M., Fendt S.M., Nalbant P, & Grüner B.M. (2021). Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis. Cell Reports, 37(8), 110056.

+ Open protocol

+ Expand

Isolation and Characterization of Muscle Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

After isolation, SCs were expanded for 6 days on collagen-coated dishes (Corning). Media was removed and dishes were rinsed with PBS and incubated for 10 min at 37 °C. Subsequently, the dish was firmly knocked against a hard surface in order to release the cells. The PBS was collected and trypsin was added for 2 min in order to release the remaining cells. All staining was performed on ice and in flow cytometry staining buffer consisting of PBS supplemented with 5 % FBS and 5 % BSA. Briefly, cells were blocked with Fc block (5 µg/ml, BD Biosciences) and stained with a PE-conjugated Syndecan-4 antibody (1 µg/ml, BD Biosciences) and an unconjugated M-cadherin primary antibody (1 µg/ml, R&D systems) followed by an AF 594 secondary antibody (1 µg/ml, Invitrogen). A dead cell dye (7-AAD, BD biosciences) was used to discriminate between live and dead cells. Flow cytometry data was collected on a LSRII flow cytometer (BD Biosciences, Franklin Lakes, NJ) and analyzed using FlowJo software.

Bauer A., Gu L., Kwee B., Li A., Dellacherie M., Celiz A.D, & Mooney D.J. (2017). Hydrogel substrate stress-relaxation regulates the spreading and proliferation of mouse myoblasts. Acta biomaterialia, 62, 82-90.

+ Open protocol

+ Expand

Differentiation of PC-12 Cells into Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat pheochromocytoma (PC-12) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were grown in suspension in RPMI-1640 (ATCC) with 10% heat-inactivated horse serum (Gibco, Grand Island, NY, USA) and 5% fetal bovine serum (Gibco) at 37 °C and 5% CO₂. To differentiate the cells into neuronal cells [26 (link)], 5 × 10⁴ cells were grown for 10 days on collagen-coated dishes (Corning, Corning, NY, USA) in RPMI-1640 with 1% heat-inactivated horse serum and nerve growth factor (NGF) (100 ng/mL, Alomone Labs, Jerusalem, Israel). The medium was changed every two days to fresh differentiation medium. NGF was removed overnight before the day of the experiment. Neuronal morphology and neurite outgrowth were assessed via microscopy before proceeding.

Rothschadl M.J., Sathyanesan M, & Newton S.S. (2023). Synergism of Carbamoylated Erythropoietin and Insulin-like Growth Factor-1 in Immediate Early Gene Expression. Life, 13(9), 1826.

+ Open protocol

+ Expand

Isolation and Culture of Primary Murine Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary hepatocytes were isolated from mouse livers as described previously.11 (link) Mouse livers were perfused in situ with EGTA solution, followed by pronase (P147; Sigma) solution and collagenase D (11088882001; Roche, Basel, Switzerland) solution at 37°C. After perfusion, the livers were treated with protease and collagenase D solution and centrifuged at 300 ×g for 3 m at 4°C to isolate hepatocytes. Primary hepatocytes were cultured in Dulbecco’s modified Eagle’s medium (10564029; Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum, insulin (I9278; Sigma), dexamethasone (D4902; Sigma), and penicillin-streptomycin (15070063; Gibco) in collagen-coated dishes (354236; Corning, New York, NY, USA).

Zhang N., Yang P., Li Y., Ouyang Q., Hou F., Zhu G., Zhang B., Huang J., Jia J, & Xu A. (2024). Serum Iron Overload Activates the SMAD Pathway and Hepcidin Expression of Hepatocytes via SMURF1. Journal of Clinical and Translational Hepatology, 12(3), 227-235.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!