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Biotin anti mouse cd106

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Biotin anti-mouse CD106 is a lab equipment product used in flow cytometry applications. It is a monoclonal antibody that specifically binds to the mouse CD106 antigen, also known as VCAM-1 (Vascular Cell Adhesion Molecule-1). The antibody is conjugated with biotin, a small molecule that can be detected using streptavidin-based detection systems.

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Lab products found in correlation

Apc anti mouse cd45, by BioLegend (2 mentions) Streptavidin pe cy7, by BioLegend (2 mentions) Apc anti mouse cd31, by BioLegend (2 mentions) Sytox green, by Thermo Fisher Scientific (2 mentions) Pacific blue anti mouse ly 6a e, by BioLegend (2 mentions)

Close spelling variants of this product

CD106 (8 citations) Anti-mouse CD106 (2 citations) Anti-CD106 (2 citations)

2 protocols using biotin anti mouse cd106

1

Isolation of Skeletal Muscle Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hindlimb muscles from 3-month-old C56BL/6J wild-type mice were dissociated into single cells by enzymatic digestion and live cells were isolated by FACS. Skeletal MuSCs were sorted following described methods (Liu et al., 2015 (link)). Briefly, hindlimb muscles from 3-month-old adult wild-type mice were minced and digested with collagenase for 1 h and MuSCs were released from muscle fibers by further digesting the muscle slurry with collagenase/dispase for an additional 30 min. After filtering out the debris, cells were incubated with the following primary antibodies: biotin anti-mouse CD106 (anti-VCAM1, BioLegend 105704; 1:75), PE/Cy7 Streptavidin (BioLegend 405206; 1:75), Pacific Blue anti-mouse Ly-6A/E (anti-Sca1, BioLegend 108120; 1:75), APC anti-mouse CD31 (BioLegend 102510; 1:75) and APC anti-mouse CD45 (BioLegend 103112; 1:75). Satellite cells were sorted by gating VCAM1-positive, Pacific Blue-labeled Sca1-negative, and APC-labeled CD31/CD45-negative cells. SYTOX Green (ThermoFisher Scientific S7020; 1:30,000) was used as a counterstain.
Khateb M., Perovanovic J., Ko K.D., Jiang K., Feng X., Acevedo-Luna N., Chal J., Ciuffoli V., Genzor P., Simone J., Haase A.D., Pourquié O., Dell’Orso S, & Sartorelli V. (2022). Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution. Cell reports, 40(7), 111219.
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2

Isolation of Skeletal Muscle Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hindlimb muscles from 3-month-old C56BL/6J wild-type mice were dissociated into single cells by enzymatic digestion and live cells were isolated by FACS. Skeletal MuSCs were sorted following described methods (Liu et al., 2015 (link)). Briefly, hindlimb muscles from 3-month-old adult wild-type mice were minced and digested with collagenase for 1 h and MuSCs were released from muscle fibers by further digesting the muscle slurry with collagenase/dispase for an additional 30 min. After filtering out the debris, cells were incubated with the following primary antibodies: biotin anti-mouse CD106 (anti-VCAM1, BioLegend 105704; 1:75), PE/Cy7 Streptavidin (BioLegend 405206; 1:75), Pacific Blue anti-mouse Ly-6A/E (anti-Sca1, BioLegend 108120; 1:75), APC anti-mouse CD31 (BioLegend 102510; 1:75) and APC anti-mouse CD45 (BioLegend 103112; 1:75). Satellite cells were sorted by gating VCAM1-positive, Pacific Blue-labeled Sca1-negative, and APC-labeled CD31/CD45-negative cells. SYTOX Green (ThermoFisher Scientific S7020; 1:30,000) was used as a counterstain.
Khateb M., Perovanovic J., Ko K.D., Jiang K., Feng X., Acevedo-Luna N., Chal J., Ciuffoli V., Genzor P., Simone J., Haase A.D., Pourquié O., Dell’Orso S, & Sartorelli V. (2022). Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution. Cell reports, 40(7), 111219.
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