Anti hemagglutinin ha h3663

Human recombinant IL-8 was purchased from PeproTech (Rocky Hill, NJ). The following primary antibodies were used: anti-His antibodies (ab18184, Abcam, Cambridge, UK); anti-REEP5 (sc-133405), anti-REEP6 (sc-133410), anti-pERK (sc-7383), anti-ERK antibodies (sc-94), anti-β-actin (sc-47778) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-hemagglutinin (HA)(H3663) and anti-FLAG antibodies (F3165); phalloidin-TRITC (P1951, Sigma-Aldrich, St. Louis, MO). All reagents were from Sigma-Aldrich unless otherwise stated. Molecular weight marker (DokDo-MARK^TM, broad range) in SDS-PAGE and DNA size ladder marker (1 Kbp plus 100 bp) were from Invitrogen(Carlsbad, CA).

Mouse monoclonal anti-Flag (F1804) and anti-hemagglutinin (HA) (H3663) were obtained from Sigma-Aldrich. Anti-Myc (2278) and Atg7 (8558S) were obtained from Cell Signaling Technology. Anti-RNF5 (sc-81716), ALIX (sc-53540), and ubiquitin (sc-8017) were obtained from Santa Cruz Biotechnology. Anti-HSP90 (60318-1-Ig) was obtained from Proteintech. Rabbit anti-LC3 (PM036) was obtained from MBL. Anti-tubulin (E7-S) was obtained from the Developmental Studies Hybridoma Bank. POH1 (A9608), CD63 (A19023), SARS-CoV-2 M (A20232), and SARS-CoV-2 E (A20199) were obtained from ABclonal. SARS-CoV-2 N (40143-MM05) was obtained from Sino Biological Inc. Mouse anti-p62 (H00008878-M01) was obtained from Abnova. Goat anti-mouse IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate (A11031), goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate (A11036), Goat anti-mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (A32723), goat anti-rabbit IgG (H+L) secondary antibody, and Alexa Fluor 488 conjugate (A32731) were obtained from Thermo Fisher Scientific. Anti-Myc magnetic beads (B26301) and anti-HA magnetic beads (B26201) were obtained from Bimake. Anti-Flag M2 affinity gel (A2220) was obtained from Sigma-Aldrich. Torin1 (F6101) was obtained from Ubiquitin-Proteasome Biotechnologies.