Cd3 pe clone 145 2c11

Manufactured by Thermo Fisher Scientific

CD3-PE (clone 145-2C11) is a laboratory reagent used for the detection and analysis of CD3-positive cells in flow cytometry applications. It is a fluorescently-labeled monoclonal antibody that binds to the CD3 antigen expressed on the surface of T cells. The PE (phycoerythrin) fluorescent label allows for the visualization and quantification of CD3-positive cells in a sample.

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Lab products found in correlation

Cd3 pecy5, by BD (1 mentions) Anti human igg pe, by Jackson ImmunoResearch (1 mentions) Cd4 apc h7 clone rpa t4, by BD (1 mentions) Cd45 apcefluor780 clone 30 f11, by Thermo Fisher Scientific (1 mentions) Cd8 percp cy5.5 clone 53 67, by BD (1 mentions) Ly6g apc clone 1a8, by BioLegend (1 mentions) Ly6c percpcy5.5 clone al 21, by BD (1 mentions) Cd11b pe clone m1 70, by BioLegend (1 mentions) Lsrii flow cytometer, by FlowJo (1 mentions)

Spelling variants of this product

Clone 145-2C11 (42 citations) CD3-PE (37 citations) CD3 (clone 145-2C11, (20 citations) CD3 (145-2C11, (16 citations) Anti-CD3 PE (clone 145-2C11, (5 citations)

2 protocols using cd3 pe clone 145 2c11

Multi-Target Antibody Protocol: Optimizing Immune Responses

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ATOR-1015 (anti-CTLA-4 x anti-OX40 bsAb), anti-OX40 mAb, anti-CTLA-4 (anti-GFP x anti-CTLA-4 bsAb) and IgG1 isotype control (anti-GFP) were developed using the proprietary ALLIGATOR-GOLD® library and FIND® optimization technology (Additional file 1: Supplementary Methods). For flow cytometry analysis of human cells, the following anti-human antibodies were used: CD3-PECy5 (clone SP34–2), CD4-APC-H7 (clone RPA-T4), CD25-BV421 (clone 2A3), CD127-FITC (clone HIL-7R-M21), CD134 (OX40)-PE (clone L106) and CD152 (CTLA-4)-APC (clone BNI3) (all from BD) and anti-human IgG-PE (Jackson Immuno-Research).
For flow cytometry analysis of murine cells, the following anti-mouse antibodies were used: CD25-PerCPCy5.5 (clone PC61.5), CD45-APCeFluor780 (clone 30-F11), NK1.1-FITC (clone PK136) and Foxp3-APC (clone FJK-16 s) from eBioscience, CD3-PE (clone 145-2C11), CD3-PECy7 (clone 145-2C11), CD4-BV510 (clone RM4–5), CD4- PECy7 (clone RM4–5), CD8-PE (clone 53–6.7), CD8-PerCPCy5.5 (clone 53–6.7), CD11b-FITC (clone M1/70), CD19-FITC (clone 1D3), CD107a-APC (clone 1D4B), MHC-II-FITC (clone 2G9), Granzyme B-PE (clone GB11) and FVS450 viability stain from BD.

Kvarnhammar A.M., Veitonmäki N., Hägerbrand K., Dahlman A., Smith K.E., Fritzell S., von Schantz L., Thagesson M., Werchau D., Smedenfors K., Johansson M., Rosén A., Åberg I., Winnerstam M., Nyblom E., Barchan K., Furebring C., Norlén P, & Ellmark P. (2019). The CTLA-4 x OX40 bispecific antibody ATOR-1015 induces anti-tumor effects through tumor-directed immune activation. Journal for Immunotherapy of Cancer, 7, 103.

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Comprehensive Immune Cell Profiling in Lung Tissue

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Lung cells (1 × 10⁶ to 2 × 10⁶) were stained for surface antigens for 30 min at 4°C. Stained cells were washed and then fixed overnight in PBS containing 2% paraformaldehyde. The following antibodies were used: CD3-PE (clone 145-2C11; eBioscience), CD4-PB (clone RM4-5; eBioscience), CD8-FITC (clone 5H10-1; BioLegend), CD44-PerCPCy5.5 (clone 1M7; eBioscience), CD62L-PECy7 (clone MEL-14; BioLegend), CD19-APC (clone eBio1D3; eBioscience), Ly6G-APC (clone 1A8; BioLegend), Ly6C-PerCPCy5.5 (clone AL-21; BD Pharmingen), MHC II-FITC (clone AMS-32.1; BD Pharmingen), CD11b-PE (clone M1/70; BioLegend), and CD11c-PB (clone N418; BioLegend). Samples were run on an LSRII flow cytometer with Diva software, and the data were analyzed using FlowJo version 10.1.r7 software. The total number of cells in each gate was calculated using the total number of cells determined by the Countess automated cell counter. The gating strategy used is shown in Fig. S2 in the supplemental material.

Bhatt K., Machado H., Osório N.S., Sousa J., Cardoso F., Magalhães C., Chen B., Chen M., Kim J., Singh A., Ferreira C.M., Castro A.G., Torrado E., Jacobs WR J.r., Bhatt A, & Saraiva M. (2018). A Nonribosomal Peptide Synthase Gene Driving Virulence in Mycobacterium tuberculosis. mSphere, 3(5), e00352-18.

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