Tris-2-carboxyehyl-phosphine (TCEP), 6-mercapto-1-hexanol (99%), Trizma base (2-amino-2-(hydroxymethyl)-1,3-propanediol, magnesium chloride (MgCl2), sodium chloride (NaCl), tobramycin (Tob), ferrocene carboxylic acid, 97% (FCC), sulfuric acid (H2SO4), and 10X Tris-EDTA buffer, Dulbecco’s modified Eagle’s medium (DMEM), sodium hydroxide (NaOH), sodium acetate (NaOAC) Tetrabutylammonium hexafluorophosphate (TBAPF6), ferrocene (FC) Fetal Bovine Serum (FBS), and Protector RNase Inhibitor were all used as received from Sigma-Aldrich. Hydrogen peroxide 30%, 95% ethanol, and 10x PBS buffer were used as received (Fischer Scientific). Collagen I from rat tail was used as received (Gibco). Ambion RNaseAlert QC System was used as obtained from Thermo Fischer Scientific. SP Sepharose Fast Flow was used as received from GE Healthcare Life Sciences. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25 °C) using a Biopak Polisher Millipore ultra-purification system (Millipore, Billerica, MA). The RNA aminoglycoside aptamer sequence (5′-HSC6-CUUGGUUUAGGUAAUGAG-MB-3′ (D2 Sequence)16 (link) was purified using dual-HPLC (Biosearch Technologies, CA) and used as received.
Hydrogen peroxide 30
Manufactured by Thermo Fisher Scientific
Sourced in Canada
Hydrogen peroxide 30% is a chemical compound commonly used in various laboratory applications. It is a clear, colorless liquid with a distinctive odor. The core function of this product is to serve as an oxidizing agent, providing a source of active oxygen for a variety of chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Sp sepharose fast flow, by GE Healthcare (1 mentions)
Dual hplc, by Biosearch Technologies (1 mentions)
Collagen 1 from rat tail, by Thermo Fisher Scientific (1 mentions)
Polisher millipore ultra purification system, by Merck Group (1 mentions)
Protector rnase inhibitor, by Merck Group (1 mentions)
Ferrocene, by Merck Group (1 mentions)
Tween 20, by Thermo Fisher Scientific (1 mentions)
Formalin 10, by Merck Group (1 mentions)
Western lightning chemiluminesce nce plus, by PerkinElmer (1 mentions)
Slowfade gold antifade reagent, by Thermo Fisher Scientific (1 mentions)
Polyethylenimine (pei), by Avantor (1 mentions)
Nonidet p 40, by Merck Group (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Wisent (1 mentions)
3 protocols using hydrogen peroxide 30
1
Aminoglycoside Aptamer Purification and Characterization
2
Protein Extraction and Analysis Protocol
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Sodium orthovanadate (Na3VO4), trypsin inhibitor, PMSF, Nonidet P-40, Triton X-100, formalin 10%, aprotinin and leupeptin were obtained from Sigma-Aldrich Canada (Oakville, ON, Canada) and the Western Lightning Chemiluminescence Plus from PerkinElmer (Guelph, ON, Canada). Fetal bovine serum (FBS) and Dulbeco’s modified Eagle’s medium (DMEM) were purchased from Wisent Bioproducts (St-Bruno, QC, Canada). And Tween 20 as well as hydrogen peroxide (30%) from Fischer Scientific (Ottawa, ON, Canada). Polyethylenimine (PEI) was obtained from VWR (Mississauga, ON, Canada) and slowFadeTM Gold antifade reagent from Thermo Fisher Scientific (Waltham, MA, USA).
3
Purification and Characterization of Horse Heart Mb
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Horse
heart Mb and catalase
were purchased from Sigma-Aldrich. The Mb was oxidized to iron (III)
by ferricyanide, desalted with a PD10 column, and stored in 50 mM
sodium acetate buffer at pH 5.0. Concentrations of stocks were determined
through absorbance with a reference of A409 nm= 171 mM–1 cm–1. Hydrogen peroxide
30% was purchased from Thermo Fisher Scientific.
heart Mb and catalase
were purchased from Sigma-Aldrich. The Mb was oxidized to iron (III)
by ferricyanide, desalted with a PD10 column, and stored in 50 mM
sodium acetate buffer at pH 5.0. Concentrations of stocks were determined
through absorbance with a reference of A409 nm= 171 mM–1 cm–1. Hydrogen peroxide
30% was purchased from Thermo Fisher Scientific.
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