The SHG intensity spectrum was measured with a fully-automated homemade nonlinear microscope system as illustrated in Fig. 4c . A pulsed light with tunable wavelength was generated by a Ti:Sapphire laser system (Chameleon Ultra II, Coherent) combined with an optical parametric oscillator (Compact OPO, Coherent). The pulses wavelength was tuned from 1050 nm to 1350 nm with a duration typically around 200 fs, a repetition rate of 80 MHz and an average power at the sample of 5 mW. The beam was focused to a beam radius of 5 μm on the sample with a lens (A240TM with NA = 0.5, Thorlabs) and collected with a 100× objective (LMPlanFL N with NA = 0.8, Olympus). The sample was firmly attached on both sides and stretching was achieved by translating the two sample holders in opposite directions (precision of translation is 0.1 mm for a 15 mm rest length). The signal was focused with a convex lens (la1461 with f = 250 mm, Thorlabs) onto a sCMOS camera (Zyla 4.2, Andor) and the SH signal was separated using two high-pass filters.
A240
Manufactured by Thorlabs
Sourced in Germany
The A240TM is a micrometer-driven translational stage from Thorlabs. It provides linear motion with a travel range of 25 mm and a minimum incremental motion of 10 μm. The stage features a black anodized aluminum construction and is compatible with Thorlabs' selection of mounting accessories.
Automatically generated - may contain errors
2 protocols using a240
1
Automated Nonlinear Microscopy for SHG Imaging
2
Photoluminescence Characterization of Microfluidics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A 375 nm LED (M375L3-Mounted LED, Thorlabs, Germany) was used as
an excitation source for PL measurements. The collimated beam was
directed toward a dichroic beam splitter (Multiphoton LP-Strahlenteiler
HC 375 LP, AHF, Germany) and then focused into the microfluidic channel
using an aspheric lens (A240TM, f = 8.0 mm, NA 0.50, Thorlabs, Germany).
Emission originating from the microfluidic channel was collected by
the same lens, passed through the dichroic beam splitter, and coupled via a 10× objective (RMS10X, NA 0.25, Thorlabs, Germany)
to a fiber spectrometer (QE 65000, Ocean Optics, UK) via a 2 m long multimode fiber with a core diameter of 400 μm
(QP400-2-UV–vis, Ocean Optics, UK). The spectrometer incorporated
a 20 μm entrance slit, a 600 lines/mm grating, and a 2048-pixel
detector. The spectrometer was operated between 350 and 1100 nm, and
data were recorded using an integration time between 50 and 100 ms.
an excitation source for PL measurements. The collimated beam was
directed toward a dichroic beam splitter (Multiphoton LP-Strahlenteiler
HC 375 LP, AHF, Germany) and then focused into the microfluidic channel
using an aspheric lens (A240TM, f = 8.0 mm, NA 0.50, Thorlabs, Germany).
Emission originating from the microfluidic channel was collected by
the same lens, passed through the dichroic beam splitter, and coupled via a 10× objective (RMS10X, NA 0.25, Thorlabs, Germany)
to a fiber spectrometer (QE 65000, Ocean Optics, UK) via a 2 m long multimode fiber with a core diameter of 400 μm
(QP400-2-UV–vis, Ocean Optics, UK). The spectrometer incorporated
a 20 μm entrance slit, a 600 lines/mm grating, and a 2048-pixel
detector. The spectrometer was operated between 350 and 1100 nm, and
data were recorded using an integration time between 50 and 100 ms.
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