Diskovery platform

Manufactured by Oxford Instruments

The Diskovery Platform is a versatile lab equipment product designed for high-throughput material characterization. It utilizes cutting-edge technology to enable efficient and precise analysis of a wide range of materials. The core function of the Diskovery Platform is to provide researchers and scientists with a powerful tool for material investigation and discovery.

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Lab products found in correlation

Eclipse ti inverted microscope, by Oxford Instruments (3 mentions) Diskovery tirf borealis widefield illuminator, by Oxford Instruments (3 mentions) Perfect focus system, by Oxford Instruments (2 mentions) Apo tirf objective, by Nikon (2 mentions) Perfect focus system, by Nikon (1 mentions)

Close spelling variants of this product

Diskovery Platform/borealis widefield illuminator

4 protocols using diskovery platform

High-Resolution TIRF Microscopy Imaging

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Total internal reflection fluorescence microscopy (TIR‐FM) was performed as previously described.⁶³ Briefly, cells were mounted in PBS and imaged using a 60×, 1.49 NA APO TIRF objective (Nikon) mounted on a fully motorized Nikon Ti‐Eclipse inverted microscope with Perfect Focus System and coupled to an Andor “Diskovery TIRF/Borealis widefield illuminator” equipped with an additional 1.8× tube lens (yielding a final magnification of ×108). TIR‐FM illumination was achieved using a Diskovery Platform (Andor Technology). For live cell experiments, cells were maintained at 37°C during imaging. Imaging sequences were acquired using a sCMOS camera with 6.5 μm pixel size (pco.edge).

Lakoduk A.M., Kadlecova Z, & Schmid S.L. (2020). A functionally neutral single chain antibody to measure beta‐1 integrin uptake and recycling. Traffic (Copenhagen, Denmark), 21(9), 590-602.

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Live-cell TIRF Microscopy of Clathrin

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Total internal reflection fluorescence microscopy (TIR-FM) was performed as previously described (Loerke et al., 2009 (link)). Briefly, sCLCa expressing GFP-CLCa and sCLCb expressing GFP-CLCb H1299 and A549 cells were imaged using a 100× 1.49 NA Apo TIRF objective (Nikon) mounted on a Ti-Eclipse inverted microscope with Perfect Focus System (Nikon). TIR-FM illumination was achieved using a Diskovery Platform (Andor Technology). During imaging, cells were maintained at 37°C in RPMI supplemented with 5% fetal calf serum. Time-lapse image sequences were acquired at a penetration depth of 90 nm and a frame rate of 1Hz using a sCMOS camera with 6.5µm pixel size (pco.edge).

Chen P.H., Bendris N., Hsiao Y.J., Reis C.R., Mettlen M., Chen H.Y., Yu S.L, & Schmid S.L. (2017). Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis. Developmental cell, 40(3), 278-288.e5.

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Selective Imaging of Peripheral Endosomes

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To selectively image endosomal compartments close to the cell periphery, we adjusted the illumination of TIRF evanescent field to a theoretical penetration depth of ∼200 nm (thick TIRF). Briefly, fixed cells were mounted in PBS and imaged using a 60×, 1.49 NA APO TIRF objective (Nikon) mounted on a fully motorized Nikon Ti-Eclipse inverted microscope with Perfect Focus System and coupled to an Andor Diskovery TIRF/Borealis widefield illuminator equipped with an additional 1.8× tube lens (yielding a final magnification of 108×). TIRF illumination was achieved using a Diskovery Platform (Andor Technology). During imaging, cells were maintained at 37°C. Image sequences were acquired using a scientific CMOS camera with 6.5-µm pixel size (pco.edge).

Lakoduk A.M., Roudot P., Mettlen M., Grossman H.M., Schmid S.L, & Chen P.H. (2019). Mutant p53 amplifies a dynamin-1/APPL1 endosome feedback loop that regulates recycling and migration. The Journal of Cell Biology, 218(6), 1928-1942.

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Live Cell Imaging with TIR-FM

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Total internal reflection fluorescence microscopy (TIR-FM) was performed as previously described.^{63 (link)} Briefly, cells were mounted in PBS and imaged using a 60×, 1.49 NA APO TIRF objective (Nikon) mounted on a fully motorized Nikon Ti-Eclipse inverted microscope with Perfect Focus System and coupled to an Andor “Diskovery TIRF/ Borealis widefield illuminator” equipped with an additional 1.8× tube lens (yielding a final magnification of ×10⁸). TIR-FM illumination was achieved using a Diskovery Platform (Andor Technology). For live cell experiments, cells were maintained at 37°C during imaging. Imaging sequences were acquired using a sCMOS camera with 6.5 μm pixel size (pco.edge).

Lakoduk A.M., Kadlecova Z, & Schmid S.L. (2020). A functionally neutral single chain antibody to measure beta‐1 integrin uptake and recycling. Traffic (Copenhagen, Denmark), 21(9), 590-602.

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