Peroxidase and

Manufactured by Jackson ImmunoResearch

Peroxidase- and Alkaline Phosphatase-Conjugated Antibodies are commonly used as detection reagents in various immunoassays and immunochemical techniques. These antibodies are conjugated with enzymes that catalyze the conversion of colorless substrates into colored products, allowing for the visualization and quantification of target molecules.

Automatically generated - may contain errors

Lab products found in correlation

Mouse monoclonal anti α tubulin clone dm1a, by Merck Group (2 mentions) Anti aurora b, by Abcam (2 mentions) Mouse monoclonal anti flag clone m2, by Merck Group (2 mentions) Alexa fluor conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Mouse monoclonal anti cit k, by BD (1 mentions)

2 protocols using peroxidase and

Generation and Characterization of CHMP4C Phospho-Specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

CHMP4C phospho-specific antibodies were raised in rabbits against synthetic CHMP4C peptides encompassing residues 206–220 (TARRSRAASSQRAEEC) and containing either a phosphorylated serine at position S210 (TARRpSRAASSQRAEEC; mono-phospho CHMP4C) or phosphorylated serines at position S210, S214 and S215 (TARRpSRAApSpSQRAEEC; triphospho CHMP4C). Sera were first eluted through a column containing a non-phosphorylated peptide, and then each antibody was affinity purified using the appropriate phosphopeptide. Peptide synthesis, conjugation, rabbit immunizations, serum production and affinity purification were carried out by Generon, UK.
Other antibodies used in this study were: mouse monoclonal anti α-tubulin (clone DM1A, Sigma, T9026), chicken polyclonal anti-α-tubulin (Abcam, ab89984), mouse monoclonal anti-Flag (clone M2, Sigma, F3165), rabbit polyclonal anti-Aurora B (Abcam, ab2254), mouse monoclonal anti-Borealin (clone 1D11-MLB Life Science M147-3), rabbit polyclonal anti-KIF23/MKLP1 (clone N19, Santa Cruz Biotechnology, sc-867), rabbit polyclonal anti-CHMP4B, a gift of Sagona & Stenmark [36 (link)]. Peroxidase- and Alexa-fluor-conjugated secondary antibodies were purchased from Jackson Laboratories and Thermo, respectively.

Capalbo L., Mela I., Abad M.A., Jeyaprakash A.A., Edwardson J.M, & D'Avino P.P. (2016). Coordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesis. Open Biology, 6(10), 160248.

+ Open protocol

+ Expand

Antibody Panel for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: mouse monoclonal anti α-tubulin (clone DM1A, Sigma, T9026), chicken polyclonal anti-α-tubulin (Abcam, ab89984), mouse monoclonal anti-Flag (clone M2, Sigma, F3165), mouse monoclonal anti-CIT-K (BD Transduction Laboratories, 611377), rabbit polyclonal anti-Aurora B (Abcam, ab2254), rabbit polyclonal anti-pT232 Aurora B (Abcam, ab61074), rabbit polyclonal anti-INCENP (clone P240, Cell Signaling, 2807), rabbit polyclonal anti-pTSS INCENP (a kind gift of M.A. Lampson) [33 (link)], rabbit polyclonal anti-KIF23 (clone N19, Santa Cruz Biotechnology, sc-867), rabbit polyclonal anti-KIF14 (Bethyl Laboratories, A300–233A) and rabbit polyclonal anti-KIF20A (a kind gift of T.U. Mayer) [44 (link)]. Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and Invitrogen.

McKenzie C., Bassi Z.I., Debski J., Gottardo M., Callaini G., Dadlez M, & D'Avino P.P. (2016). Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis. Open Biology, 6(3), 160019.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!