Fluo 4 calcium assaykit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Fluo-4 Calcium Assay Kit is a fluorescence-based reagent used to measure intracellular calcium levels in cells. The kit includes the calcium-sensitive dye Fluo-4 and buffers necessary for loading the dye into cells and detecting the fluorescent signal.

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Fluo-4 (326 citations)

2 protocols using fluo 4 calcium assaykit

Multiplex Cytokine and RNA Analysis

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DMEM, FBS, penicillin, streptomycin, PBS, and other tissue culture reagents were
purchased from Gibco BRL (Grand Island, NY, USA). Emodin, poly I:C, indomethacin, Griess
reagent, and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The multiplex bead-based cytokine assay kits used for the determination of cytokine
concentration were purchased from Millipore (Billerica, MA, USA). The Fluo-4 calcium assay
kit was purchased from Molecular Probes (Eugene, OR, USA). QuantiGene Plex 2.0 Reagent
System for direct quantification of multiple RNA targets was purchased from Panomics
(Redwood City, CA, USA).

Kim Y.J., Lee J.Y., Kim H.J., Kim D.H., Lee T.H., Kang M.S., Choi Y.K., Lee H.L., Kim J., An H.J, & Park W. (2017). INHIBITORY EFFECT OF EMODIN ON RAW 264.7 ACTIVATED WITH DOUBLE STRANDED RNA ANALOGUE POLY I:C. African Journal of Traditional, Complementary, and Alternative Medicines, 14(3), 157-166.

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Diospyrin-mediated Cellular Responses

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Dulbecco’s modified Eagle’s medium (DMEM), FBS, penicillin, streptomycin, PBS, and other cell culture reagents were purchased from Millipore (Billerica, MA, USA). Diospyrin was isolated from Diospyros lotus by Dr. Inamullah Khan. Multiplex cytokine assay kits were purchased from Millipore. The Fluo-4 calcium assay kit was supplied by Molecular Probes (Eugene, OR, USA). Real-time RT-PCR kits were ordered from Bio-Rad (Hercules, CA, USA). Phospho-p38 MAPK Antibody (T180/Y182) (eBioscience 17-9078-42) and Mouse IgG2b kappa Isotype Control (eBioscience 12-4732-81) were obtained from Life Technologies Corporation (Carlsbad, CA, USA). All other solutions for flow cytometric analysis were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Shahidullah A., Lee J.Y., Kim Y.J., Halimi S.M., Rauf A., Kim H.J., Kim B.Y, & Park W. (2020). Anti-Inflammatory Effects of Diospyrin on Lipopolysaccharide-Induced Inflammation Using RAW 264.7 Mouse Macrophages. Biomedicines, 8(1), 11.

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