Radioactive arachidonoyl ethanolamide 1 3h 3h aea

Radioactive arachidonoyl ethanolamide[1-³H] ([³H]-AEA) was obtained from American Radiolabeled Chemicals, Inc (St Louis, MO, USA). (R)(-)-Flurbiprofen and (S)(+)-Flurbiprofen were purchased from Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). Ovine COX-1 (cat. no. 60100), human recombinant COX-2 (cat. no. 60122), COX-2 polyclonal antibody (rabbit anti-mouse, cat #: 160106), arachidonic acid, 2-AG, AEA and URB597 (cyclohexylcarbamic acid 3′-carbamoylbiphenyl-3-yl ester) were purchased from the Cayman Chemical Co. (Ann Arbor, MI, USA). Substrates were dissolved in ethanol or DMSO as appropriate. Polyclonal goat anti-rabbit immunoglobulin/HRP was obtained from Dako (Glostrup, Denmark). Protease inhibitor cocktail set III was obtained from Merck Millipore (Darmstadt, Germany). For the lipid quantification experiments, the following native and deuterated standards were purchased from the Cayman Chemical Co.: AEA, 2-AG, PEA, OEA, DEA, LEA, SEA, 2-LG, AEA-d⁸, 2-AG-d⁸, PEA-d⁴, SEA-d³, OEA-d⁴, PGF_2α, PGE₂, TXB₂, PGD₂, 12(13)-EpOME, 9(10)-DiHOME, 12(13)-DiHOME, 11-HETE, 12-HETE, 15-HETE, 9-HODE, 13-HODE, 12-HEPE, 12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid (CUDA), 12(13)-DiHOME-d⁴, 12(13)-EpOME-d⁴, 9-HODE-d⁴, PGE₂-d⁴ and PGD₂-d⁴. 9,10,13-TriHOME and 9,12,13-TriHOME were obtained from Larodan (Sweden, Malmö). For list of lipid abbreviations, see S1 Table.

Radioactive arachidonoyl ethanolamide[1-³H] ([³H]-AEA) was obtained from American Radiolabeled Chemicals, Inc (St Louis, MO, USA). (R)(-)-Ibuprofen and (S)(+)-ibuprofen, were purchased from Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). The enantiomers of Flu-AM1 (structures, see Fig 1) were synthesised as described elsewhere [26 (link)]). AEA and URB597 (cyclohexylcarbamic acid 3′-carbamoylbiphenyl-3-yl ester) were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Carprofen was obtained from Sigma-Aldrich Inc, St. Louis, (MO, U.S.A.). Substrates were dissolved in ethanol or DMSO as appropriate.