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Variskan flash microplate reader

Manufactured by Thermo Fisher Scientific
Sourced in Japan

The Variskan® Flash microplate reader is a versatile instrument designed for rapid and accurate detection of various assays in microplate format. It measures absorbance, fluorescence, and luminescence with high sensitivity and precision. The Variskan® Flash provides a reliable and efficient solution for a wide range of applications in academic, industrial, and clinical research settings.

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2 protocols using variskan flash microplate reader

1

CCK-8 Assay for MDA-MB-231 Cell Viability

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Cell viability was determined using a CCK-8 assay according to the manufacturer’s protocol. MDA-MB-231 cells were seeded at a density of 5 × 103 cells/well in a 96-well culture plate for 24 h after which the medium was changed and BPTS (5, 10, 15 μg/mL) was added and cultured for a further for 24, 48 or 72 h. 20 μl CCK-8 reagent was added to each well and incubated for 4 h. The optical density at 450 nm was measured using a Variskan® Flash microplate reader (Thermo Scientific Fisher, Inc.).
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2

Liver Lipid Profiling via Enzymatic Assay

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The total triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured for serum and supernatant of the homogenized liver following the instructions of enzymatic assay kits (Nanjing Jiancheng, Nanjing, China), using Hitachi 7080 automatic biochemical analyzer (Hitachi, Ltd., Tokyo, Japan) and Thermo Scientific Variskan Flash microplate reader (Thermo Fisher Scientific, MA, United States).
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