Alexa flour 488 conjugated secondary antibody

Manufactured by Jackson ImmunoResearch

Sourced in United Kingdom

Alexa Fluor 488-conjugated secondary antibody is a fluorescently labeled antibody used for detection in various immunological techniques. It is designed to bind and detect primary antibodies from specific host species. The Alexa Fluor 488 dye attached to the secondary antibody emits green fluorescence when excited by a suitable light source, enabling visualization and quantification of the target.

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Lab products found in correlation

Lsm 700 laser scanning confocal system, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Ab22048, by Abcam (1 mentions) Ab181153, by Abcam (1 mentions) Anti citrate synthase, by Cell Signaling Technology (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) Ab46545, by Abcam (1 mentions)

Close spelling variants of this product

Alexa-488 conjugated secondary antibody Alexa 488 secondary antibody Alexa Flour 488 Alexa 488

2 protocols using alexa flour 488 conjugated secondary antibody

Immunofluorescence Quantification of γH2AX

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Cells were fixed for 10 min with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS for 20 min and blocked with donkey serum (PBS with 0.3% triton, donkey serum 1:100). Cells were then stained overnight at 4 °C with anti-ɣH2AX antibody (Cell Signaling, Danvers, MA, USA; #9718, 1:400), followed by incubation for 1 h at room temperature with Alexa Flour 488-conjugated secondary antibody (Jackson Immunoresearch, Cambridge, UK; #711–545-152, 1:500) and 0.2 μg/ml 4′,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, Rehovot, Israel). Images were collected using LSM 700 laser scanning confocal system (Zeiss, Gottingen, Germany). Mean number of foci per nucleus was determined using the FIJI software with the BioVoxxel plugin.

Fishman H., Monin R., Dor-On E., Kinzel A., Haber A., Giladi M., Weinberg U, & Palti Y. (2023). Tumor Treating Fields (TTFields) increase the effectiveness of temozolomide and lomustine in glioblastoma cell lines. Journal of Neuro-Oncology, 163(1), 83-94.

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Immunofluorescence Staining of Brain Sections

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Paraformaldehyde fixed frozen brain sections or BMVECs grown on slides were subsequently washed with TBS followed by treatment with 0.2% Triton X-100 for 3 minutes. After washing, samples were blocked by 5% BSA for 1 hour at room temperature. Slides were then incubated with primary antibodies like anti-IREB2 (iron responsive element binding protein 2) (ab181153, Abcam), anti-4 hydroxynonenal (anti-4HNE) (ab46545, Abcam), anti-TLR4 (toll like receptor 4) (ab22048, Abcam) and anti-citrate synthase (14309, Cell Signaling) at a 1:100 dilution in 0.2% BSA at 4°C overnight. Cells were washed and incubated with AlexaFlour 488 conjugated secondary antibody (Jackson Immuno Research Laboratories, Inc., West Grove, PA) at a 1:400 dilution at room temperature for 1 hour. Negative control slides were incubated with 0.2% BSA in place of the primary antibody. Slides were imaged on Axiovert 200 microscope (Carl Zeiss MicroImaging, Thornwood, NY).

Abdul Y., Li W., Ward R., Abdelsaid M., Hafez S., Dong G., Jamil S., Wolf V., Johnson M.H., Fagan S.C, & Ergul A. (2020). Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis. Translational stroke research, 12(4), 615-630.

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