Human lung adenocarcinoma A549 cells and breast cancer cell MCF7 were obtained from Genechem (Shanghai, Genechem Co. Shanghai, China). Human hepatoma cell line MHCC-97H were obtained from Botian (Xi’an, China). A549 cells were cultured in F12K (Hyclone) with 10% fetal bovine serum (FBS, Biological Industries, Israel). MCF7 and MHCC-97H cells were cultured in DMEM medium (Hyclone) with 10% fetal bovine serum (Biological Industries, Israel). Brefeldin A treatment: Cells were treated with BFA (MedChemExpress, Shanghai, China) dissolved in DMSO for 12 hr at various doses. Tunicamycin treatment: Cells were treated with Tunicamycin (MedChemExpress, Shanghai, China) dissolved in DMSO for 12 hr at various doses. Midiv-1 treatment: Cells were treated with Midiv-1 (MedChemExpress, Shanghai, China) dissolved in DMSO for 12 hr at various doses.
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