P38 inhibitor sb203580

Colistin (C-4461), lipopolysaccharides (LPS) (L4391), and carboxylate-modified fluorescent microspheres (L4655, 1.0 µm in diameter) were purchased from Sigma, USA. p38 inhibitor (SB203580) was purchased from MedChemExpress (USA). Fetal bovine serum (FBS) was obtained from Gibco (USA), and bovine serum albumin (BSA) was supplied by Roche (Switzerland). RPMI-1640 medium and phosphate buffered saline (PBS) were provided by HyClone (USA). DMSO and thiazolyl blue (MTT) were purchased from AMRESCO (USA); trypsin and penicillin-streptomycin were obtained from TBD Science (China). Phospho-p38 MAP kinase (Thr180/Tyr182) antibody (#9211) was supplied by Cell Signaling (USA). Horse radish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody was provided by Solarbio (China), and antibody against β-actin was obtained from Thermo Fisher (USA). Enzyme-linked immunosorbent assay (ELISA) kits for various cytokines were purchased from R&D Systems (USA).

Antibody for β-actin was purchased from Sigma (St. Louis, MO). Antibodies for hypoxia inducible factor-1α (HIF-1α), heatshockprotein70 (HSP70), phosphorylated p38 (Tyr182) were purchased from Cell Signaling Technology (Beverly, MA). TF was purchased from Santa Cruz Biotechnology (Dallas, Texas, USA.). Secondary antibodies were purchased from Sigma (St. Louis, MO). L-OHP was purchased from Jiangsu heng rui medicine (Lianyungang, China). Gelatin was purchased from Amresco (Solon, OH, USA). Zymogram renaturing buffer and Zymogram developing buffer were purchased from Novex (Carlsbad, CA, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Gibco, and other cell culture media and supplements were purchased from HyClone (Logan, UT, USA). Coomassie brilliant blue G250 was purchased from Beyotime (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from Sigma Chemical Co (St. Louis, MO). All other chemicals were purchased from Sigma Chemical Co (St. Louis, MO). Hirudin was purchased from Genscript (Nanjing, China). p38 inhibitor SB 203580 was purchased from MedChemExpress (NJ, USA).

Aluminum oxide (Al₂O₃, 20 nm and 26.93 μm), titanium oxide (TiO₂, 20 nm and 1 μm), iron oxide (Fe₂O₃, 50 nm and 1 μm) and silicon dioxide (SiO₂, 20 nm and 2.34 μm) were purchased from Shanghai YunFu Nanotechnology Co., Ltd. (Shanghai, China), Fuchen Chemical Reagent Co., Ltd. (Tianjin, China), and Aladdin (Shanghai, China). Monoclonal anti-dinitrophenol (DNP)-IgE antibody, DNP-human serum albumin (HSA), and 4-nitrophenyl N-acetyl-β-D-glucosaminide were obtained from Sigma–Aldrich (St. Louis, USA). Antibodies as against phosphor-JNK, JNK, phosphor-LYN, LYN, phosphor-Plcγ1, Plcγ1, phosphor-P38, P38, phosphor-ERK1/2, ERK1/2, NF-κB, and β-actin were obtained from Cell Signaling Technology Inc. (Danvers, MA). ERK inhibitor U0126-EtOH and p38 inhibitor SB203580 were purchased from MedChem Express (Monmouth Junction, NJ, USA), PE-anti-mouse CD117 (c-kit), APC-anti-mouse FcϵRIα, and FITC-anti-mouse IgE antibodies were purchased from Biolegend (San Diego, CA, USA). Recombinant interleukin (IL)-3 and stem cell factor (SCF) were purchased from PeproTech (Cranbury, NJ, USA). Evans blue and formamide were obtained from Dalian Meilun Biotechnology Co. Ltd. (Dalian, China). The ovalbumin (OVA; grade V) was purchased from Sigma Company (St. Louis, USA), and aluminum hydroxide was purchased from Thermo Fisher Scientific (Massachusetts, USA).