Intracellular CFU were determined by serially diluting and plating cell lysates from BMDMs that had been challenged with bacteria for 1 or 2 hours and then treated with 100 μg/ml gentamicin for 15 min. For bacterial survival time course, BMDMs were challenged with UPEC for 2 hours, treated with 100 μg/ml gentamicin for 1 hour, and then 10 μg/ml gentamicin for the remaining time stated. For imaging analysis, cells were stained with rabbit anti-E.coli (1:500, US Biologicals, Swampscott, MA)21 (link) and rat anti-LAMP1 (1:50, clone ID4B; Developmental Studies Hybridoma Bank, Iowa City, IA)21 (link), and antigen-antibody complexes were detected with anti-rabbit Alexa Fluor-488-conjugated secondary antibody and anti-rat Alexa-594-conjugated secondary antibody (both at 1:500, Invitrogen; Carlsbad, CA). Images were obtained with a Zeiss Apotome microscope.
Anti rat alexa 594 conjugated secondary antibody
Manufactured by Thermo Fisher Scientific
The Anti-rat Alexa-594-conjugated secondary antibody is a laboratory reagent used to detect and visualize rat primary antibodies in various immunoassays and imaging applications. It is a fluorescently labeled antibody that specifically binds to rat immunoglobulins, allowing for the identification and localization of target proteins or cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti rat alexa 594 conjugated secondary antibody
1
Intracellular CFU Determination for UPEC
2
Intracellular CFU Determination for UPEC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intracellular CFU were determined by serially diluting and plating cell lysates from BMDMs that had been challenged with bacteria for 1 or 2 hours and then treated with 100 μg/ml gentamicin for 15 min. For bacterial survival time course, BMDMs were challenged with UPEC for 2 hours, treated with 100 μg/ml gentamicin for 1 hour, and then 10 μg/ml gentamicin for the remaining time stated. For imaging analysis, cells were stained with rabbit anti-E.coli (1:500, US Biologicals, Swampscott, MA)21 (link) and rat anti-LAMP1 (1:50, clone ID4B; Developmental Studies Hybridoma Bank, Iowa City, IA)21 (link), and antigen-antibody complexes were detected with anti-rabbit Alexa Fluor-488-conjugated secondary antibody and anti-rat Alexa-594-conjugated secondary antibody (both at 1:500, Invitrogen; Carlsbad, CA). Images were obtained with a Zeiss Apotome microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!