Modular p2 analyzer

Fasting blood samples were drawn from each participant at both visits in the morning. Samples were transferred to the laboratory within 2 hours, divided into single-use aliquots, and frozen at -80 °C. All serum measurements were performed in 1 batch after completion of the entire study with fully automated equipment. Fasting glucose, cholesterol, high-density lipoprotein (HDL) cholesterol, and triglyceride levels were determined using the Modular P2 analyzer (Roche, Almere, the Netherlands), fasting serum insulin using immunoassay by Immulite 2500 (DPC, Los Angeles, CA, USA). Glycated hemoglobin was determined by high performance liquid chromatography (Primus Ultra2, Trinity Biotech Company, Kansas City, MO, USA). C-reactive protein was determined using a high-sensitivity immunoassay (COBAS Integra, Roche, IN, USA). Low-density lipoprotein (LDL) cholesterol was calculated using the Friedewald formula in participants without hypertriglyceridemia [20 (link)].

Serum was collected non-fasted at the study center. For the present study the following serum levels were included in the analyses: glucose, insulin, total cholesterol, LDL cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides, and 25-hydro-xyvitamin D. These metabolic serum measures have previously been shown to be different between the offspring and controls [13 (link)-16 , 21 (link)], and have been previously associated with APOE genotype [7 (link), 8 (link), 25 (link), 26 (link)]. All serum levels were measured using fully automated equipment. The levels of glucose, total cholesterol, HDL cholesterol, and triglycerides were determined with the Modular P2 analyzer from Roche (Almere, the Netherlands). Insulin was measured with the Immulite 2500 from DPC (Los Angeles, CA, USA). An electrochemiluminescence immunoassay was used to determine the level of 25-hydroxyvitamin D using a Cobas e411 analyzer from Roche Diagnostics (Almere, the Netherlands). The level of LDL cholesterol in serum was estimated using the formula by Friedewald [27 (link)]. For this, individuals with a triglyceride level >4.5 mmol/L were excluded.