Mhh nb 11

Cell lines were grown according to the suppliers’ instructions at 37 °C in a 5%-CO₂ humidified atmosphere. CHP-134, IMR-32, KELLY, LAN-1, SK-N-BE2 and −DZ were obtained from ECACC (Salisbury, UK). CHP-126, MHH-NB-11 and SIMA were obtained from DSMZ (Braunschweig, Germany). CHP-212 was obtained from ATCC. STA-NB-1, −7 and −10 were kindly provided by Dr. Peter F. Ambros (CCRI, Vienna, Austria). All cell lines were used at early passages (n = 3) to avoid the insurgence of any alteration, and all were checked for mycoplasma and other potential infections. All cell lines were checked against the Database of Cross-contaminated or Misidentified Cell Lines (http://iclac.org/), and none were found to have been previously flagged as cross-contaminated or misidentified.

ES lines (MHH‐ES1, RD‐ES, SK‐ES1, SK‐N‐MC, and TC‐71), neuroblastoma lines (CHP126, MHH‐NB11, SHSY5Y, and SIMA), and pediatric human B‐cell precursor leukemic lines (cALL2, NALM6, and 697) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). ES line VH64 was kindly provided by Marc Hotfilder (Münster University, Münster, Germany); osteosarcoma lines (HOS, HOS‐58, MG‐63, MNNG, SaOS, SJSA01, U2OS, and ZK‐58) by Jan Smida and Michaela Nathrath, Institute of Pathology and Radiation Biology (HMGU, Neuherberg, Germany). A673 was purchased from ATCC (LGC Standards, Teddington, UK). SB‐KMS‐KS1 and SB‐KMS‐MJ1 are ES cell lines that were established in our laboratory (Grunewald et al., 2012; Richter et al., 2009). Retrovirus packaging cell line PT67 was obtained from Takara Bio Europe/Clontech (Saint‐Germain‐en‐Laye, France). Cells were maintained in a humidified incubator at 37 °C in 5–8% CO₂ atmosphere in RPMI 1640 or DMEM (both Life Technologies, Carlsbad, CA, USA) containing 10% heat‐inactivated fetal bovine serum (Biochrom, Berlin, Germany) and 100 μg·mL⁻¹ gentamicin (Life Technologies). Cell lines were checked routinely for purity (e.g., EWS‐FLI1 translocation product, surface antigen or HLA phenotype) and mycoplasma contamination.