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Il 18

Manufactured by InvivoGen
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Sourced in Germany, United States

IL-18 is a lab equipment product that can be used for research applications. It functions as an interleukin-18 protein, which is a cytokine involved in immune system regulation. Further details on its intended use are not available.

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Lab products found in correlation

Il 15, by Miltenyi Biotec (2 mentions) Il 12, by Thermo Fisher Scientific (2 mentions) K562 cell, by American Type Culture Collection (1 mentions) Il 12, by BioLegend (1 mentions) Interleukin 2 (il 2), by R&D Systems (1 mentions) Cxcl1 kc, by R&D Systems (1 mentions) Cxcl2 mip 2, by R&D Systems (1 mentions) Human il 18, by R&D Systems (1 mentions) Mouse il 1β, by BD (1 mentions) Cxcl8, by BD (1 mentions) Il 18, by R&D Systems (1 mentions) Nigericin, by InvivoGen (1 mentions) Il 1β, by InvivoGen (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Il 1α, by Thermo Fisher Scientific (1 mentions) Il 33, by Thermo Fisher Scientific (1 mentions) Pan caspase inhibitor z vad fmk, by R&D Systems (1 mentions) Il 36, by Thermo Fisher Scientific (1 mentions) Cell activation cocktail, by BioLegend (1 mentions) Brefeldin a solution 1000x, by BioLegend (1 mentions)

Close spelling variants of this product

IL-18 HEK-Blue assay (4 citations) HEK-Blue IL-18 cells (3 citations)

6 protocols using il 18

1

Characterizing HLA-E+ K562 Cells

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Anti-human monoclonal antibodies (mAb) were used for flow and mass cytometry (supplemental methods, Table S4). Endotoxin-free, recombinant human (rh) IL-12 (Biolegend), IL-15 (Miltenyi), and IL-18 (InVivo Gen) were used in these studies. K562 cells [American Type Culture Collection (ATCC), CCL-243] were obtained in 2008, viably cryopreserved, and maintained for <2 months at a time in continuous culture according to ATCC specifications. K562 were authenticated in 2015 using single-nucleotide polymorphism analysis and were found to be exactly matched to the K562 cells from the Japanese Collection of Research Bioresources, German Collection of Microorganisms, and Cell Cultures (DSMZ), and ATCC databases (Genetic Resources Core Facility at Johns Hopkins University). HLA-E+ K562 were a gift from Dr. Bhattacharya. These cells were generated using the AAVS1-EF1a donor plasmid containing the coding sequence for human HLA-E. The K562 were electroporated using a Bio-Rad Gene Pulse electroporation system. HLA-E+ cells were sorted to >98% purity.
Berrien-Elliott M.M., Cashen A.F., Cubitt C.C., Neal C.C., Wong P., Wagner J.A., Foster M., Schappe T., Desai S., McClain E., Becker-Hapak M., Foltz J.A., Cooper M.L., Jaeger N., Srivatsan S.N., Gao F., Romee R., Abboud C.N., Uy G.L., Westervelt P., Jacoby M.A., Pusic I., Stockerl-Goldstein K.E., Schroeder M.A., DiPersio J, & Fehniger T.A. (2020). Multidimensional analyses of donor memory-like NK cells reveal new associations with response after adoptive immunotherapy for leukemia. Cancer discovery, 10(12), 1854-1871.
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2

Immune Modulation with Cytokine Proteins

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hIL18/IL12/TxM protein (molecular weight 245 kDa), lot #305-86 and #305-110, and N-803 protein (molecular weight 92.36 kDa), lot #01062016, were manufactured and purified at Altor BioScience (Miramar, FL). Endotoxin-free, rhIL-12 (BioLegend, San Diego, CA), IL-15 (Miltenyi, Bergisch Gladbach, Germany), IL-18 (InvivoGen, San Diego, CA), and IL-2 (R&D Systems, Minneapolis, MN) were used in these studies.
Cubitt C.C., McClain E., Becker-Hapak M., Foltz J.A., Wong P., Wagner J.A., Neal C.C., Marin N.D., Marsala L., Foster M., Schappe T., Soon-Shiong P., Lee J., Berrien-Elliott M.M, & Fehniger T.A. (2022). A novel fusion protein scaffold 18/12/TxM activates the IL-12, IL-15, and IL-18 receptors to induce human memory-like natural killer cells. Molecular Therapy Oncolytics, 24, 585-596.
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3

Quantification of Inflammatory Cytokines

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Mouse IL-1β (BD Biosciences, North Ryde, NSW, Australia), IL-18 (R&D Systems, In Vitro Technologies Pty Ltd., VIC, Australia), Cxcl1/KC (R&D Systems), Cxcl2/MIP2 (R&D Systems), human IL-18 (R&D Systems) and CXCL8 (BD Biosciences) were quantified by ELISA, as per the manufacturers’ instructions. Production of bioactive IL-18 by AGS cells was also measured using the HEK-Blue™ IL-18 reporter cell line system (InvivoGen, San Diego, CA, USA).
Tran L.S., Ying L., D’Costa K., Wray-McCann G., Kerr G., Le L., Allison C.C., Ferrand J., Chaudhry H., Emery J., De Paoli A., Colon N., Creed S., Kaparakis-Liaskos M., Como J., Dowling J.K., Johanesen P.A., Kufer T.A., Pedersen J.S., Mansell A., Philpott D.J., Elgass K.D., Abud H.E., Nachbur U., Croker B.A., Masters S.L, & Ferrero R.L. (2023). NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice. Nature Communications, 14, 3804.
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4

Bone Marrow-Derived Macrophage Infection Assay

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Bone marrow-derived macrophages (BMMs) were cultured for 7 days in alpha-MEM supplemented with10% fetal bovine serum (FBS) and recombinant M-CSF (30 ng/ml). 105 BMMs were plated in a 24 well plate 12–16 hr before stimulation. 10403S WT Lm, ΔactA mutant and LLO deleted (Δhly) mutant strains were grown to mid-log phase, washed with PBS, and added at a ratio of 10 to 1 cell (MOI 10). Nigericin (20 uM), IL-1β (100 ng/ml; Invivogen), or IL-18 (100 ng/ml; InvivoGen) were added 1hr before infection. 100 uM ATPγS, 10 U/ml apyrase, 100 uM ADPβS, 100 uM adenosine, and 10 U/ml adenosine deaminase were added 30 min before infection. All were purchased from Millpore Sigma. To measure phagocytosis, cells were lysed with 0.1% Triton X-100 for enumerating initial uptake for 30 min. Two hr post-infection, gentamycin was added to kill the extracellular bacteria, then two hr later, cells were lysed with 0.1% Triton X-100 and bacterial CFUs were enumerated by serial dilution.
Jeong Y.H., Walsh M.C., Yu J., Shen H., Wherry E.J, & Choi Y. (2020). Mice lacking the purinergic receptor P2X5 exhibit defective inflammasome activation and early susceptibility to Listeria monocytogenes. Journal of immunology (Baltimore, Md. : 1950), 205(3), 760-766.
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5

Cytokine-Induced Cell Activation Assay

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IL-15 at (Peprotech #200-15), IL-12 (Peprotech #200-12), IL-18 (Invivogen #rcyec-hil18), IL-1α (Peprotech #200-200-01A), IL-1β ((Peprotech #200-01B), IL-33 (Peprotech #200-33), IL-36 (Peprotech #200-36), Pan-Caspase Inhibitor Z VAD FMK (R&D Systems #FMK001), Brefeldin A Solution (1000X) (BioLegend #420601), Granzyme B Inhibitor II, Calbiochem (EMD Millipore #368055-1MG), Cell Activation Cocktail (BioLegend #423302) were used.
Berjis A., Muthumani D., Aguilar O.A., Pomp O., Johnson O., Finck A.V., Engel N.W., Chen L., Plachta N., Scholler J., Lanier L.L., June C.H, & Sheppard N.C. (2024). Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation. Nature Communications, 15, 3937.
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6

Modulation of Cytokine-Induced Cell Signaling

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ATP, A438079, BAPTA‐AM, TMI‐I (WAY‐171318) and marimastat were from Sigma‐Aldrich. Caspase 3 inhibitor I and pan‐caspase inhibitor (Z‐VAD‐FMK) were from Merck Millipore. Recombinant human IL‐15, IL‐12 and IL‐21 were supplied by PeProtech, whereas IL‐2 (Proleukin®) was from Novartis and IL‐18 from Invivogen.
Baroja‐Mazo A., Peñín‐Franch A., Lucas‐Ruiz F., de Torre‐Minguela C., Alarcón‐Vila C., Hernández‐Caselles T, & Pelegrín P. (2022). P2X7 receptor activation impairs antitumour activity of natural killer cells. British Journal of Pharmacology, 180(1), 111-128.
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