To transiently inhibit miR-21 expression, hsa-miR-21 mirVana™ miRNA inhibitor (Applied Biosystems) or hsa-miR-21 mirVana® mimics (Applied Biosystems) were used to transfect HNSCC cells, as described previously [19 (link)]. Verification of transfection efficiency was conducted using the mirVana™ miRNA Mimic Negative Control (Applied Biosystems) and mirVana™ miRNA Inhibitor Negative Control (Applied Biosystems), respectively. Forward transfections were conducted by mixing miRNA mimic or inhibitor (70 nM) with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) and Opti-MEM I (Invitrogen) and applying the mixture to cells at 24 h after plating. A series of in vitro assays was conducted after 48–72 h of incubation.
Hsa mir 21 mirvana mirna inhibitor
Manufactured by Thermo Fisher Scientific
The Hsa-miR-21 mirVana™ miRNA inhibitor is a synthetic oligonucleotide designed to target and inhibit the expression of the human microRNA (miRNA) hsa-miR-21. miRNAs are small, non-coding RNA molecules that play a role in the regulation of gene expression. The Hsa-miR-21 mirVana™ miRNA inhibitor is intended to be used as a research tool to study the function of hsa-miR-21 in cellular and biological processes.
Automatically generated - may contain errors
2 protocols using hsa mir 21 mirvana mirna inhibitor
1
Transient miR-21 Inhibition in HNSCC
2
Transient Inhibition of miR-21 in HNSCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To transiently inhibit miR-21 expression, hsa-miR-21 mirVana™ miRNA inhibitor (Applied Biosystems) or hsa-miR-21 mirVana® mimics (Applied Biosystems) were used to transfect HNSCC cells, as described previously [18] . Veri cation of transfection e ciency was conducted using the mirVana™ miRNA Mimic Negative Control (Applied Biosystems) and mirVana™ miRNA Inhibitor Negative Control (Applied Biosystems), respectively. Forward transfections were conducted by mixing siRNA oligonucleotides (70 nM) with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) and Opti-MEM I (Invitrogen) and applying the mixture to cells at 24 h after plating. A series of in vitro assays was conducted after 48-72 h of incubation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!