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3 protocols using anti cd140b pe

1

Multi-Marker Flow Cytometry Profiling

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Antibodies anti-CD31 PE-Cy7, anti-CD18 PE or APC, anti-CD51 FITC, anti-CD64 FITC, anti-CD14 FITC, PE or PerCP-Cy5.5, anti-CD66abce FITC, anti-CD34 VioBlue®, anti-CD38 FITC, anti-CD44 PE, anti-CD45 VioBlue® or APC-Vio770, anti-CD3 FITC or PerCP-Cy5.5, anti-CD20 FITC or PerCP-Cy5.5, anti-CD15 FITC, anti-CD16 PerCP-Cy5.5, anti-HLA-DR VioBlue®, anti-CD86 VioBlue®, anti-CD80 VioBlue®, anti-CD90 FITC and PerCP-Cy5.5, anti-CD133 APC, anti-CD138 PE, anti-CD140a PE, anti-CD146 FITC, and anti-MSCA-1 PE were from Miltenyi Biotec. Anti-STRO-1 AlexaFluor® 647, anti-CD73 PE-Cy7, anti-CD105 PerCP-Cy5.5, anti-CD140b PE, anti-CD56 APC, anti-CD309 PerCP-Cy5.5, anti-CD115, anti-CD144 PE, anti-SSEA-3 FITC, and anti-SSEA-4 FITC were from BioLegend (San Diego, CA). Anti-CD271 PE, anti-CD11b PE, anti-CD11c PE, and anti-CD36 APC were from BD Biosciences (San Jose, CA).
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Flow cytometry characterization of C-MSCs

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C-MSC were blocked with 5% rat serum and stained respectively with conjugated antibodies, including anti-CD105-APC (BioLegend, San Diego, CA, USA), anti-CD31-APC (BD Biosciences, San Jose, CA, USA), anti-CD45-APC (BD Biosciences), anti-CD140b-PE (BioLegend), or isotype-matched control antibody (BD Biosciences). Flow cytometry analysis of cultured C-MSC was performed with a BD LSRII flow cytometer and BD FACSDiva™ software (8.02, BD Biosciences, San Jose, CA, USA).
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3

Cardiac Mesenchymal Stem Cell Immunophenotyping

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Cardiac mesenchymal stem cells were first blocked with 5% rat serum (Sigma) and stained, respectively, with conjugated antibodies, including anti-CD105-APC (BioLegend), anti-CD140b-PE (BioLegend), or isotype-matched control antibody (BD Biosciences). Flow cytometry analysis of cultured C-MSC was performed with a BD LSRII flow cytometer from Augusta University.
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