Human umbilical cord MSCs (hUC-MSCs) were purchased from Zhongkeweixin Biotechnology (Chengdu, China) and maintained in complete medium (DMEM-LG with 100 mL/L FBS and 100 µg/mL penicillin and 50 µg/mL streptomycin) in an incubator supplied with a humidified atmosphere of 50 mL/L CO2 at 37 °C. For flow cytometric detection of surface antigens, hUC-MSCs were detached from flasks. Then, hUC-MSCs were washed and resuspended at a concentration of 1 × 106 viable cells/mL for 30 min in the dark at 2-8 °C in PBS containing saturation concentrations (1:100 dilution) of the following conjugated mouse or rat monoclonal antibodies against human antigens (BD Biosciences, San Jose, CA, United States): CD34-APC-A, CD45-FITC-A, CD90-PE, CD73-PCA, CD105 PE-A Isotype control, CD19-FITC Isotype control, CD11b-APC Isotype control, and HLA-DR-PC5.5 Isotype control. Cells were washed twice and resuspended in 500 μL of PBS for flow cytometry (FACS Aria, BD Biosciences) using FLOWJO TM software (TreeStar, Inc., Ashland, OR, United States).
Huc mscs
Manufactured by BD
Sourced in United States
HUC-MSCs are a type of primary human umbilical cord-derived mesenchymal stem cells. They are multipotent cells capable of differentiating into various cell types such as osteoblasts, chondrocytes, and adipocytes.
Automatically generated - may contain errors
2 protocols using huc mscs
1
Phenotypic Characterization of Umbilical Cord MSCs
2
Characterization of hUCMSCs Differentiation
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The hUCMSCs were purchased from Haixing Biosciences (Suzhou, Jiangsu, China). The hUCMSCs were cultured in accordance with a previously described method[45 (link)]. Afterward, the adipogenic, osteogenic, chondrogenic capacity of these cells was performed. Passages 3–7 of hUCMSCs were cultured in osteogenic, adipogenic or chondrogenic differentiation medium (Cyagen, China, HUXUC-9004) as described by manufacturer. Oil red O staining, Alizarin red staining and Alcian blue staining were used to evaluate adipogenesis, osteogenesis, and chondrogenesis. Detection of hUCMSCs surface markers by a FACSVerse instrument (BD Bioscience, San Jose, CA, USA) is performed as follows. The hUCMSCs were stained with human anti-CD14, anti-CD19, anti-CD34, anti-CD45, anti-CD73, anti-CD90, anti-CD105, or anti-HLA-DR. Identical concentrations of PE-conjugated mouse IgG isotype antibodies were used as negative controls (all from BD Biosciences). Data were analyzed using the software FlowJo V10 (FlowJo).
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