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Quantikine elisa kit for human il 6

Manufactured by R&D Systems
Sourced in United States

The Quantikine ELISA Kit for human IL-6 is a quantitative sandwich immunoassay designed to measure human interleukin-6 (IL-6) levels in cell culture supernates, serum, and plasma. It utilizes the quantitative sandwich enzyme immunoassay technique to determine the concentration of human IL-6 in a sample.

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2 protocols using quantikine elisa kit for human il 6

1

Quantification of IL-6 Production

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IL-6 protein was determined in cell culture supernatants using the Quantikine Elisa Kit for human IL-6 (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Samples were analysed in triplicates. IL-6-production rate was calculated with the formula, whereby T2 and T1 were two adjacent time points: (IL-6T2-IL-6T1)/(T2-T1).
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2

IL-6 Measurement in HERV-K dUTPase Cells

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In PAECs treated with HERV-K dUTPase, the IL-6 protein was measured using the Quantikine ELISA kit for human IL-6 (R&D Systems) according to the manufacturer’s protocol.
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