M csf

Manufactured by RayBiotech

M-CSF is a cytokine that functions as a growth factor for monocytes and macrophages. It is involved in the differentiation and proliferation of these cells.

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Lab products found in correlation

M csf, by Apexbio (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Il 10, by RayBiotech (1 mentions) Il 13, by RayBiotech (1 mentions) Interleukin 4 (il 4), by RayBiotech (1 mentions) Rantes, by RayBiotech (1 mentions) Tnf α, by RayBiotech (1 mentions)

2 protocols using m csf

Isolation and Polarization of Rat Bone Marrow-Derived Macrophages

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The femur was isolated from euthanized Wistar male rats and marrow was flushed into a cold PBS solution containing 2% fetal bovine serum (FBS). Mechanically dissociated cells were incubated on ice with three volume of 0.8% NH₄Cl solution to remove red blood cells. 10⁶ cells/cm² were seeded in tissue culture plates (Corning Constar) and cultured in Iscove's Modified Dulbecco's Medium (IMDM, Gibco) supplemented with 10% FBS, 1% v/v penicillin-streptomycin (Life Technologies) 10ng/ml M-CSF (RayBiotech). After 7 days, bone marrow-derived macrophages (MΦ) were polarized to inflammatory M1 with IMDM, 1% v/v penicillin-streptomycin, 10ng/ml M-CSF supplemented with 100ng/ml TNFα (ApexBio) and 100ng/ml IFNγ (RayBiotech). The non-inflammatory BMDM-M2 profile was induced by supplementing the culture medium with 20ng/ml IL-4, 20ng/ml IL-10 and 20ng/ml IL13 (RayBiotech).

Biemmi V., Milano G., Ciullo A., Cervio E., Burrello J., Dei Cas M., Paroni R., Tallone T., Moccetti T., Pedrazzini G., Longnus S., Vassalli G, & Barile L. (2020). Inflammatory extracellular vesicles prompt heart dysfunction via TRL4-dependent NF-κB activation. Theranostics, 10(6), 2773-2790.

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Cytokine Analysis in Stroke Model

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For cytokine analysis, 2 months old males (6 animals per group) were used. At 1, 4 or 7 days after stroke the animals were anesthetized and their blood flushed out with 20ml cold PBS via transcardial perfusion. Then, the brains were harvested and cut to 1mm thick coronal sections. After a quick TTC staining, infarct core (approximate 2 by 2 mm) was dissected out and frozen with liquid nitrogen. Since we found that the most anterior sections, containing the striatum area, produced the most comparable size between the cortical strokes in each type of model, we limited the cytokine analysis to the first half of the brain. For cytokine analysis, brain tissue was subject to multiplex ELISA analysis through the Quantibody Mouse Cytokine Array for the following cytokines/chemokines: bFGF, BLC, CD30L, eotaxin, eotaxin-2, FasL, G-CSF, GM-CSF, ICAM, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p40, IL-13, IL-15, IL-17, IL-21, KC, leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1a, MIP-1g, PF-4, RANTES, TARC, TCA-3, TNF RI, TNF RII, TNFα (RayBiotech Inc.).

Cotrina M.L., Lou N., Tome-Garcia J., Goldman J, & Nedergaard M. (2016). DIRECT COMPARISON OF MICROGLIAL DYNAMICS AND INFLAMMATORY PROFILE IN PHOTOTHROMBOTIC AND ARTERIAL OCCLUSION EVOKED STROKE. Neuroscience, 343, 483-494.

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