Hmga2

Manufactured by Merck Group

Sourced in United States

HMGA2 is a laboratory equipment product from Merck Group. It is a protein that plays a role in the regulation of gene expression. The core function of HMGA2 is to act as an architectural transcription factor, which means it can bind to DNA and modify its structure, thereby influencing the activity of genes.

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Lab products found in correlation

3 protocols using hmga2

Western Blot Analysis of Cellular Proteins

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Cells were harvested and washed in ice-cold 1X phosphate-buffered saline (Life Technologies, Grand Island, NY). Cell pellets were lysed, separated on polyacrylamide gels and immunoblotted, as described (39 (link)). INI1, HMGA2, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin and cleaved poly (ADP-ribose) polymerase (PARP) expression were analyzed using the antibodies to the following: INI1 (Sigma-Aldrich), HMGA2 (Cell Signaling Technology, Inc., Beverly, MA), GAPDH (Research Diagnostics, Inc., Flanders, NJ), β-actin (Santa Cruz Biotechnology, Inc., Dallas, TX), and cleaved PARP (Cell Signaling Technology, Inc.). Primary antibodies were detected using goat anti-mouse and goat anti-rabbit antibodies conjugated to horseradish peroxidase (KPL, Gaithersburg, MD). Secondary antibodies were detected using Western Lightning Plus ECL (PerkinElmer, Waltham, MA). Densitometry analysis was performed using Image J software (40 (link)).

Kaur H., Hütt-Cabezas M., Weingart M.F., Xu J., Kuwahara Y., Erdreich-Epstein A., Weissman B.E., Eberhart C.G, & Raabe E.H. (2015). The Chromatin Modifying Protein HMGA2 Promotes Atypical Teratoid Rhabdoid Cell Tumorigenicity. Journal of neuropathology and experimental neurology, 74(2), 177-185.

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Western Blot Analysis of Cellular Proteins

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Proteins were extracted from HO8910 and A2780 cells cultured for 30 min in a 37°C atmosphere containing 5% CO₂. The extracted proteins were separated by sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) and electrophoretically transferred onto nitrocellulose membranes (Millipore, USA). Standard WB analyses were performed using antibodies specific for CTGF (Santa Cruz, CA, USA), CYR61 (Santa Cruz, USA), FN1 (Sigma), HMGA2 (Sigma), ASAP3 (Sigma, USA), ERBB3 (Sigma, USA), IL-6 (Sigma, USA), IL-1 (Sigma, USA), JUN (Invitrogen, USA), MAP2K8 (Sigma, USA), MMP13 (Sigma, USA), NPNT (Invitrogen, USA), ODC1 (Invitrogen, USA), VEGFC (Sigma, USA) and GAPDH (Santa Cruz, USA). Immunoreactions were visualized using a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (Santa Cruz, CA, USA) and detected by enhanced chemiluminescence. BandScan software was used to analyze the grayscale values.

ZHOU T., FU P., CHEN D, & LIU R. (2023). Transformer 2β regulates the alternative splicing of cell cycle regulatory genes to promote the malignant phenotype of ovarian cancer. Oncology Research, 31(5), 769-785.

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Molecular Pathways in Liver Cancer

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The HCC cell lines 97L, HepG2, Hep3B, 7721, and Huh7, the 293T cells, and the normal human hepatocyte L02 cells were all obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China). Cell lines were cultured in humidified atmosphere with 5% CO₂ in Dulbecco’s Modified Eagle Media (Invitrogen) plus 3% fetal bovine serum (FBS, Invitrogen) and 100 μg/mL streptomycin (Invitrogen). The antibodies for HMGA2 (no.HPA039076), E-cadherin (no.5085), GAPDH (no.AMAB91153), GSK3β (no.SAB5300175), STAT3 (no.S5933), pSTAT3 (pTyr⁷⁰⁵, no.AB4300033), and ubiquitin (no.SAB1306582) were obtained from Sigma. BclxL (no.PA5-21676) and SNAIL1 (no.14-9859-82) antibodies were purchased from Invitrogen. The proteasome inhibitor MG132 was obtained from Sigma (no.M8699).

Li O., Li Z., Tang Q., Li Y., Yuan S., Shen Y., Zhang Z., Li N., Chu K, & Lei G. (2018). Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 and MiR-4516. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 8510-8523.

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