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3 protocols using aluminum chloride hexahydrate

1

Probiotic Formulation Characterization and Evaluation

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The caffeic acid, keracyanin chloride, kuromanin chloride, Folin-Ciocalteau, and quercetin used in the experiment were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aluminum chloride hexahydrate and potassium acetate were purchased from Junsei Chemical (Tokyo, Japan). Citric acid, sodium alginate, mannitol, and poloxamer 188 were purchased from Daejung (Seoul, Republic of Korea). Whey protein isolate, lecithin, and ascorbyl palmitate were purchased from ESfood (Gumpo, Republic of Korea). Lacticaseibacillus rhamnosus (L. rhamnosis), Pediococcus pentosaceus (P. pentosaceus), Enterococcus faecalis (E. faecalis). Escherichia coli (E. coli), and Streptococcus aureus (E. aureus) were purchased from ATCC (American Type Culture Collection). Man-Rogasa-shape (MRS) and brain heart infusion (BHI) medium was purchased from Solarbio (Shanghai, China).
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2

Bioactive Compound Evaluation in Food

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S Food (Gumpo, Korea) provided the whey protein isolate, lecithin, and ascorbyl palmitate used in the extrudates. The source of the citric acid was Daejung (Sihung, Korea). We purchased isoquercitrin, rutin, quercetin, and caffeic acid from Sigma-Aldrich (St. Louis, MO, USA). Potassium acetate and aluminum chloride hexahydrate were acquired from Junsei Chemical (Tokyo, Japan). The American Type Culture Collection (ATCC) provided the following microorganisms: Lacticaseibacillus rhamnosus (L. rhamnosus), Escherichia coli (E. coli), Streptococcus aureus (S. aureus), and Enterococcus faecalis (E. faecalis). Brain heart infusion (BHI) and Man-Rogasa-Shape (MRS) were acquired from Solarbio (Shanghai, China).
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3

Comprehensive Reagent Procurement Guideline

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Chemicals, reagents, and standards were procured from Sigma-Aldrich (St. Louis, MO, USA). Anhydrous sodium carbonate and acetic acid were acquired from Ducksan Pure Chemicals (Ansan, Republic of Korea), while sodium hydroxide and aluminum chloride hexahydrate were procured from Junsei Chemical (Tokyo, Japan) and Samchun Pure Chemical (Pyeongtaek, Republic of Korea), respectively. Acetic acid and anhydrous sodium carbonate were purchased from Ducksan Pure Chemicals (Ansan, Republic of Korea).
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