Mice were anesthetized with 1.5–2% isoflurane and blood was collected by intra-cardiac puncture. Serum was isolated from blood after 30-min room temperature incubation and centrifugation. Aspartate transaminase activity (BioAssay Systems), HMGB1 (LSBio), iron (Abcam), hepcidin (eLabScience), NGAL (ENZO Life Sciences), hemopexin (Alpha Diagnostic), haptoglobin (Alpha Diagnostic), and ferritin (Kamiya Biomed) were measured in the serum according to manufacturers' protocols.
Hepcidin
Manufactured by Elabscience
Sourced in United States
Hepcidin is a peptide hormone produced by the liver that regulates iron homeostasis in the body. It acts as a key regulator of iron absorption, distribution, and recycling, playing a crucial role in maintaining appropriate iron levels.
Automatically generated - may contain errors
Lab products found in correlation
Hmgb1, by LSBio (1 mentions)
Pth 1 84, by Quidel (1 mentions)
Synergy htx multi mode reader, by Agilent Technologies (1 mentions)
Ferritin, by Abcam (1 mentions)
Sod activity assay kit, by Nanjing Jiancheng (1 mentions)
Osteocalcin, by Cloud-Clone (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
Dimension rxl max integrated chemistry system, by Siemens (1 mentions)
6 protocols using hepcidin
1
Serum Biomarker Analysis in Mice
2
Serum Biomarker Measurement in Mice
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Blood was collected from anesthetized mice maintained at 2% isoflurane via cardiac puncture and collected in serum separation tubes incubated for 30 min at room temperature. Serum was isolated by centrifugation and subsequent chemistry analysis performed using enzyme-linked immunosorbent assays. Serum intact PTH 1-84 (Quidel Corporation, San Diego, CA) and hepcidin (Elabscience, Houston, TX), levels were measured using a BioTek Synergy HTX Multi-Mode Reader (BioTek Instruments, Inc., Highland Park, MO) at wavelengths specified by manufacturer protocol. Serum phosphate and iron levels were analyzed by the Animal Histopathology and Laboratory Medicine Core, University of North Carolina-Chapel Hill, Chapel Hill, NC. Serum creatinine levels were performed by the UAB O’Brien Center using liquid chromatography-tandem mass spectrometry (UAB-UCSD O’Brien Center Bioanalytical Resource Core, Birmingham, AL).
3
Serum Analysis of Bone and Oxidative Markers
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Serum samples were collected to analyze hepcidin (Elabscience, Wuhan, China), ferritin (Abcam, Cambridge, UK), osteogenic marker: osteocalcin (Cloud-Clone corp, Houston, US), and osteoclast marker: C-terminal telopeptide of type 1 collagen (CTX; Cloud-Clone corp) levels by enzyme-linked immunosorbent assays. Serum oxidative stress markers, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by using the MDA assay kit (Jiancheng, Nanjing, China) and SOD activity assay kit (Jiancheng), respectively. The optical density (OD) was measured at wavelengths of 532 nm and 450 nm, respectively. All the procedures were performed according to the manufacturers' instructions.
4
Hepcidin Quantification in Cell Media
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5
Comprehensive Iron and Inflammation Profiling
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Plasma iron, ferritin, transferrin, total iron binding capacity (TIBC), and high-sensitivity C-reactive protein (hs-CRP) levels were determined on Siemens Dimension RXL Max Integrated Chemistry System (Erlangen, Germany) by using regents supplied by the manufacturer. Transferrin saturation (TSAT) was calculated as (iron [μg/dL]/transferrin [μg/dL]) × 70.9.
14 Plasma interleukin (IL)-6 (R&D Systems, Europe) and hepcidin (Elabscience Biotechnology Co., Ltd) levels were measured by enzyme-linked immunosorbent assay. The detection limits for IL-6 and hepcidin were 0.039 pg/mL and 0.156 ng/mL, respectively. Both intra and inter-assay coefficients of variability were less than 10%. All measurements were conducted in duplicate.
14 Plasma interleukin (IL)-6 (R&D Systems, Europe) and hepcidin (Elabscience Biotechnology Co., Ltd) levels were measured by enzyme-linked immunosorbent assay. The detection limits for IL-6 and hepcidin were 0.039 pg/mL and 0.156 ng/mL, respectively. Both intra and inter-assay coefficients of variability were less than 10%. All measurements were conducted in duplicate.
6
Serum Biomarkers of Bone Metabolism
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Serum samples were collected and analyzed for hepcidin (Elabscience, TX, USA), serum ferritin (Abcam), osteogenic marker (P1NP) and osteoclast marker (CTX) (Immunodiagnostic Systems Ltd, UK) levels by enzymelinked immunosorbent assays (ELISA). All ELISA assays were performed according to manufacturer instructions.
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