α cortisol antibody and cortisol hormone hydrocortisone

Polyamide substrates were ordered from GE Healthcare Life Sciences (Piscataway, NJ, USA) with 0.2 μm pore size. The linker molecule Dithiobis [Succinimidyl Propionate] (DSP) and its solvent Dimethyl Sulfoxide (DMSO) is ordered from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The α-cortisol antibody and cortisol hormone (Hydrocortisone) was ordered from Abcam (Cambridge, MA, USA). IL-1β antigen was bought from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The antibody was diluted in 1X phosphate buffered saline (PBS, Thermo Fisher Scientific Inc., Waltham, MA, USA). Both cortisol hormone and IL-1β are diluted using synthetic sweat. The synthetic sweat was prepared as per the recipe stated in Table 2 of M.T. Mathew et al.26 . The pH range is varied by varying the concentration of the components. Human sweat was purchased from Lee biosolutions Inc. (St. Louis, MO, USA), where it was collected from single human donor with pH ~ 4–5. No preservatives have been added to this product and it was stored unfiltered at below −20 °C.

0.2 μm pore size polyamide substrates were purchased from GE Healthcare Life Sciences (Piscataway, NJ, USA). Dithiobis [Succinimidyl Propionate] (DSP), its solvent Dimethyl Sulfoxide (DMSO), and 150 mM Phosphate Buffered Saline (PBS) were ordered from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The α-cortisol antibody and cortisol hormone (Hydrocortisone) were ordered from Abcam (Cambridge, MA, USA). Single donor human sweat was purchased from Lee biosolutions Inc. (St. Louis, MO, USA). Molybdenum Disulfide (MoS₂) Crystal was purchased from SPI supplies (West Chester, PA, USA) and was diluted in Milli-Q deionized water (18 MΩ).