Hep-2 cells were cultured in 96-well plates (Corning, NY, USA) in 200 µL of total culture medium at a concentration of 1×104 cells/well. Subsequently, the cells were divided into six groups with further six sub-groups in each group. The cells in these six groups were then exposed to the photosensitizer m-THPC (HY-16488, MedChem Express, NJ, USA) at concentrations of 0.1, 0.5, 1, 2, 5, and 10 µM, respectively, and incubated in conditions devoid of light at 37°C with 5% CO2 in an incubator for 24 hrs after seeding. After 24 hrs, the cells in six sub-groups were exposed to laser of a wavelength of 650 nm (Changchun Institute of Optics, Fine Mechanics & Physics, Chinese Academy of Sciences, Changchun, China) with energy delivered at variable doses (0, 0.1, 0.5, 1, 2, and 5 J/cm2), respectively. The control group was administered no treatment and was only cultured in total culture medium. Upon three rinses with phosphate buffer saline (PBS), fresh culture medium was added to the cells. After 24 hrs, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was performed in order to measure the cellular viability. The experiments on each m-THPC concentration and laser energy dose were performed in triplicates.
M thpc
Manufactured by MedChemExpress
Sourced in United States
M-THPC is a laboratory product manufactured by MedChemExpress. It is a chemical compound used for research purposes. The core function of M-THPC is to serve as a research tool for scientific investigations.
Automatically generated - may contain errors
3 protocols using m thpc
1
Photodynamic Therapy Optimization for Hep-2 Cells
2
Photosensitizer Uptake and Cytotoxicity in SCC7 Cells
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SCC7 cells were cultured in black 96-well microplates with a clear glass bottom (Corning, USA) at a density of 6,000 cells/well. After reaching a confluence of 60–70%, the cells were washed twice with PBS and then incubated with m-THPC (MedChem Express, NJ, USA) of different concentrations (ranging from 0.5 to 10 µM) in serum-free medium in the dark for 24 h. SCC7 cells without treatment were used as a negative control. Intake of m-THPC by the SCC7 cells was detected using an inverted fluorescence emission microscope (IX71, Olympus, Japan) and quantified using a microplate reader (FLUOstar Omega, BMG LABTECH GmbH, Ortenberg, Germany). The fluorescence excitation was set at 460 nm and the emission was monitored at 650 nm. The fluorescence intensity was corrected for autofluorescence background (n = 5). CCK-8 assay kit (Sangon Biotech, Shanghai, China) was performed to detect cell viability of SCC7 cells according to the manufacturer’s instructions and the optical density was measured at 450 nm with microplate reader.
3
Photodynamic Therapy Optimization with m-THPC and VP
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The chemicals m-THPC and VP were purchased from MedChemExpress (Monmouth Junction, NJ, USA). They were dissolved in DMSO as a 10 mmol/L stock solution and stored at −80 °C, protected from light. Working solutions of m-THPC were prepared fresh at various concentrations (0, 0.375, 0.75, 1.5, 3.0, 6.0, and 12.0 μmol/L) and working solutions of VP were prepared fresh at various concentrations (0, 0.375, 0.75, 1.5, 3.0, and 6.0 μmol/L), which were activated by a halogen lamp in cell experiments, a laser power density of 10 mW/cm2 and laser energy densities of 3 J/cm2.
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