Gentamicin (Abbott, India), PVA (Sigma Aldrich, India), Escherichia coli (E.coli) (National Collection of Industrial Microorganisms (NCIM), Pune), L6 myoblast cells (National Centre for Cell Science (NCCS), Pune, India), Dulbecco's modified Eagle's medium (Himedia, India), Fetal bovine serum (Gibco, India). All other chemicals used were of analytical grade. In vivo experiment was carried out in C. L. Baid Metha College of Pharmacy, Chennai, India. Animal use and the experimental protocols were approved by the institutional animal ethics committee (IAEC).
L6 myoblast cells
Manufactured by National Centre for Cell Science
Sourced in India
L6 myoblast cells are a type of skeletal muscle cells derived from rat muscle tissue. They are commonly used as an in vitro model system to study muscle cell biology and differentiation. The L6 cells exhibit the characteristic features of myoblasts, which are the precursor cells that can differentiate into mature muscle fibers.
Automatically generated - may contain errors
2 protocols using l6 myoblast cells
1
In Vivo Assessment of Gentamicin-PVA Efficacy
2
Culturing Rat Skeletal Muscle Myoblasts
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L6 myoblast cells (rat
skeletal muscle) were procured from the National Centre for Cell Sciences
(NCCS), Pune, India. Cells were cultured and maintained in Dulbecco’s
modified Eagle’s medium (DMEM) (4.5 g/L) with 10% heat-inactivated
fetal bovine serum (FBS), 1% antibiotic–antimycotic, cultured
in a T-75 cm2 flask, and kept at 37 °C in an incubator
with 5% atmospheric CO2 and observed daily at an inverted
microscope Leica DMi1.
skeletal muscle) were procured from the National Centre for Cell Sciences
(NCCS), Pune, India. Cells were cultured and maintained in Dulbecco’s
modified Eagle’s medium (DMEM) (4.5 g/L) with 10% heat-inactivated
fetal bovine serum (FBS), 1% antibiotic–antimycotic, cultured
in a T-75 cm2 flask, and kept at 37 °C in an incubator
with 5% atmospheric CO2 and observed daily at an inverted
microscope Leica DMi1.
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