Glycosylated hemoglobin (HbA1c) was measured using a DCA Vantage Analyzer (Siemens Medical Solutions Diagnostics, Tarrytown, NY). To measure serum triglycerides, plasma samples as well as glycerol standards (Sigma-Aldrich, St Louis, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics) and concentration was determined using the colorimetric method on a Bio-Rad 680 XR (Hercules, CA, USA). Plasma non-esterified fatty acid (NEFA) was measured using a NEFA kit (WAKO, Osaka, Japan). Serum creatinine was measured using the Architect C16000 Clinical Chemistry Analyzer (Abbott Laboratories, Abbott Park, Ill, USA).
Triacylglycerol reagent
Manufactured by Roche
Sourced in United States
The Triacylglycerol reagent is a laboratory product designed to measure the concentration of triglycerides in biological samples. It is a key component in the analysis of lipid metabolism and can be used in various clinical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Glycerol standard, by Merck Group (4 mentions)
Nefa kit, by Fujifilm (3 mentions)
Alanine transaminase colorimetric activity assay kit, by Cayman Chemical (2 mentions)
Architect c16000 clinical chemistry analyzer, by Abbott (1 mentions)
Dca vantage analyzer, by Siemens (1 mentions)
Insulin mouse elisa kit, by Abnova (1 mentions)
4 protocols using triacylglycerol reagent
1
Biochemical Markers in Metabolic Health
2
Quantifying Metabolic Markers in Mice
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Plasma insulin concentration was measured using an Insulin (mouse) ELISA Kit (Abnova, Taiwan) according to the manufacturer's instructions. Samples were analysed in duplicate, and the intra-assay coefficient of variance was below 10%.
Liver lipids were extracted using the Folch method 26 , as previously described 24 (link) . Plasma, liver extracts and glycerol standards (Sigma-Aldrich, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics, Basel, Switzerland) using an in-house assay 24 (link) .
Plasma nonesterified free fatty acid (NEFA) concentrations were measured using a NEFA kit (WAKO, Osaka, Japan).
Liver lipids were extracted using the Folch method 26 , as previously described 24 (link) . Plasma, liver extracts and glycerol standards (Sigma-Aldrich, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics, Basel, Switzerland) using an in-house assay 24 (link) .
Plasma nonesterified free fatty acid (NEFA) concentrations were measured using a NEFA kit (WAKO, Osaka, Japan).
3
Evaluating Liver Lipid and Insulin Resistance
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The plasma activity of Alanine Transaminase (ALT) was measured using the Alanine Transaminase Colorimetric Activity Assay Kit (Cayman Chemical, MI, USA) according to the manufacturer's instructions. Plasma insulin concentration was measured by ELISA (Abnova, Taiwan) according to the manufacturer's instructions. Liver lipids were extracted using the Folch method 14 , as previously described 10 . Plasma, liver extracts and glycerol standards (Sigma-Aldrich, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics, Basel, Switzerland) using an in-house assay 13 . Plasma non-esterified free fatty acid (NEFA) concentrations were measured using a NEFA kit (WAKO, Osaka, Japan). The insulin resistance index Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) was calculated as insulin (µU/mL) x glucose (mM)/22.5.
4
Measurement of Liver Lipids and Function
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Plasma ALT activity was measured using the Alanine Transaminase Colorimetric Activity Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s instructions. Plasma insulin concentration was measured by ELISA (Abnova, Taiwan) according to the manufacturer’s instructions. Liver lipids were extracted using the Folch method [19 (link)]. Briefly, liver samples were homogenised in a chloroform:methanol (2:1) mixture. After agitation, the samples were washed with 0.6% saline solution and centrifuged at low speeds. The lower organic phase was then extracted and dried. Plasma, liver extracts and glycerol standards (Sigma-Aldrich, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics, Basel, Switzerland) using an in-house assay [18 (link)].
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