Aβ25-35 powder was dissolved and sonicated for 10 min in 5 M Tris-HCl buffer (pH 7.4) at 4 °C and aggregated at 37 °C for 16 h. Then SH-SY5Y-MOCK cells were incubated for 24 h with 10 µM and 1 µM Aβ. Peroxidase activation was measured using the AmplexTM Red Peroxidase Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. Tris-HCl served as control.
Amplex red peroxidase kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Amplex™ Red Peroxidase Kit is a fluorescent detection reagent used to measure hydrogen peroxide (H2O2) production or peroxidase activity. The kit utilizes the Amplex Red reagent, which reacts with H2O2 in the presence of peroxidase to produce the highly fluorescent product resorufin.
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Lab products found in correlation
3 protocols using amplex red peroxidase kit
1
Amyloid-beta Neurotoxicity Assay
2
Amplex Red Peroxidase Assay
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Peroxidase activation was measured using an AmplexTM Red Peroxidase Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. Cells were seeded in 96-well plates and incubated for 24 h.
3
Peroxidase Activity Measurement in SY5Y Cells
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Measurement of peroxidase activity in SY5Y cells was achieved using the AmplexTM Red Peroxidase Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to its protocols. Experiments were performed on cells sown into 96-well plates that were incubated with 10 µg/mL WE or ctrl for 24 h.
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